Immune-protective effectiveness of seasonal influenza split vaccine against H7N9 influenza virus infection in mice
Received:October 15, 2013  
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DOI:10.3969/j.issn.1005-4847.2014.01.009
KeyWord:H7N9 influenza virus;Seasonal influenza split vaccine;Immune-protective effectiveness;Mice
                          
AuthorInstitution
吕琦 中国医学科学院, 北京协和医学院, 医学实验动物研究所, 卫生部人类疾病比较医学重点实验室, 国家中医药管理局人类疾病动物模型三级实验室, 北京
鲍琳琳 中国医学科学院, 北京协和医学院, 医学实验动物研究所, 卫生部人类疾病比较医学重点实验室, 国家中医药管理局人类疾病动物模型三级实验室, 北京
许黎黎 中国医学科学院, 北京协和医学院, 医学实验动物研究所, 卫生部人类疾病比较医学重点实验室, 国家中医药管理局人类疾病动物模型三级实验室, 北京
邓巍 中国医学科学院, 北京协和医学院, 医学实验动物研究所, 卫生部人类疾病比较医学重点实验室, 国家中医药管理局人类疾病动物模型三级实验室, 北京
李枫棣 中国医学科学院, 北京协和医学院, 医学实验动物研究所, 卫生部人类疾病比较医学重点实验室, 国家中医药管理局人类疾病动物模型三级实验室, 北京
袁静 中国医学科学院, 北京协和医学院, 医学实验动物研究所, 卫生部人类疾病比较医学重点实验室, 国家中医药管理局人类疾病动物模型三级实验室, 北京
于品 中国医学科学院, 北京协和医学院, 医学实验动物研究所, 卫生部人类疾病比较医学重点实验室, 国家中医药管理局人类疾病动物模型三级实验室, 北京
姚艳丰 中国医学科学院, 北京协和医学院, 医学实验动物研究所, 卫生部人类疾病比较医学重点实验室, 国家中医药管理局人类疾病动物模型三级实验室, 北京
秦川 中国医学科学院, 北京协和医学院, 医学实验动物研究所, 卫生部人类疾病比较医学重点实验室, 国家中医药管理局人类疾病动物模型三级实验室, 北京
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Abstract:
      Objective To explore whether seasonal influenza split vaccine has immune protective effect on H7N9 influenza virus infection in mice. Methods Seasonal influenza split vaccine and PBS were used to immunize mice by intraperitoneal injection. 14 days later, the mice were booster immunized with A/Anhui/(H7N9) virus. Then the mice were challenged with H7N9 influenza virus in a dose of 5 LD50. H7N9 influenza virus was preincubated with mouse serum immunized with vaccine and titered by micro-neutralization and hemagglutination inhibition (HI) assay. The protective effect was judged by survival rate, body weight loss and residue virus titer in the lungs and turbinates 2 and 4 days post inoculation. Results After infection, the body weight of mice in the vaccine and model groups were declined, the survival rate of the vaccine group was 10% and all mice of the model group died. The virus titers of the turbinates in the vaccine group were significantly higher than that in model group. The neutralizing antibodies raised in the sera of all mice vaccinated with seasonal influenza split vaccine did not neutralize H7N9. Conclusion Seasonal influenza split vaccine cannot effectively protect mice against H7N9 influenza virus infection.
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