Establishment and pathological characterization of a mouse model of lung adenocarcinoma
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KeyWord:Lung; Adenocarcinoma; Adenoma; N-ethylnitrosourea; Models; Mouse
侯敏 广州医学院附属肿瘤医院肿瘤研究所,广州
戴丽军 广州医学院实验动物中心,广州
谭小军 广州医学院附属肿瘤医院病理科,广州
黄月玲 广州医学院实验动物中心,广州
高蔚樱 广州医学院附属肿瘤医院病理科,广州
吕嘉春 广州医学院公共卫生学院,呼吸疾病国家重点实验室,广州
任韵 广州医学院附属肿瘤医院肿瘤研究所,广州
周敏仪 广州医学院附属肿瘤医院肿瘤研究所,广州
朴金松 广州医学院附属肿瘤医院病理科,广州
劳淑贞 广州医学院附属肿瘤医院病理科,广州
梁成结 广州医学院实验动物中心,广州
冯瑗瑜 广州医学院实验动物中心,广州
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      Objective To establish a mouse model of lung adenocarcinoma induced by N-ethyl-N-nitrosourea (ENU) in mice and to characterize its tumor pathology. Methods BABL/c mice were used to establish the lung cancer model. On the 17th day of pregnancy, the parental female mice received intraperitoneally ENU or buffer injection, respectively. The 32-week old offsprings were sacrificed and entire lungs were harvested and processed for histopathological observation of HE stained semiserial sections. The pathological features of the lung tumors were assessed. ResultsMultiple tumors in offspring''s lungs were induced transplacentally by a single injection of ENU. The tumors were at different development stages of adenoma or adenocarcinoma. The adenocarcinomas were classified as bronchioloalveolar-like adenocarcinoma (female:5/6; male:4/6) and adenocarcinoma of various differentiation (female:4/6; male:5/6). The frequency of cancer development was 5 out of 6 mice in both sexes. The frequency of tumors which developed into malignant tumors was 16 out of 43 tumors in female and 12 out of 31 tumors in male mice. ConclusionsWe have successfully established a mouse model of lung adenocarcinoma in mouse offsprings by a single injection of ENU in pregnant female mice. The heterogeneous presentation of tumor pathology indicates the complex molecular mechanisms of its carcinogenesis.
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