Establishment and application of a mouse infection model for evaluating the virulence of Vibrio parahaemolyticus
  
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DOI:10.3969/j.issn.1005-4847.2012.04.006
KeyWord:Vibrio parahaemolyticus; Infection; Lethality; Mouse
                 
AuthorInstitution
郭李平 中国农业大学动物医学院,农业部动物流行病学与人兽共患病重点实验室,北京;军事医学科学院微生物流行病研究所,病原微生物生物安全国家重点实验室,北京
徐镔蕊 中国农业大学动物医学院,农业部动物流行病学与人兽共患病重点实验室,北京
杜德燕 军事医学科学院微生物流行病研究所,病原微生物生物安全国家重点实验室,北京
姜汉雯 军事医学科学院微生物流行病研究所,病原微生物生物安全国家重点实验室,北京
邱业峰 军事医学科学院实验动物中心,北京
周冬生 军事医学科学院微生物流行病研究所,病原微生物生物安全国家重点实验室,北京
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Abstract:
      ObjectiveTo establish a mouse infection model for evaluating the virulence of Vibrio parahaemolyticus. Methods 4-5-week old female Balb/c mice were monitored for symptoms and lethality after being infected intraperitoneally with Vibrio parahaemolyticus bacterial suspension. ResultsThe survival rate of mice after i.p. infection with a highly virulent RIMD2210633 strain (107CFU) cultivated in a high salt medium (2% NaCl) was 20-30%, while that with environmental non-virulent S251 strain was 100%. ConclusionsWe have successfully established a mouse infection model using the RIMD2210633 strain. This mouse model is suitable for evaluating the Vibrio parahaemolyticus virulence, and is used to compare the virulence of highly virulent and environmental non-virulent strains cultured in medium at different salt concentrations.
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