Evaluation of reward-relevant learning and memory behavior with operant conditioning task in rats
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KeyWord:Operant conditioning; Reward; Wistar rats;SHR rats;Learning; Memory
石哲 中国医学科学院 北京协和医学院 药用植物研究所药理毒理中心,北京
陈善广 中国航天员科研训练中心,北京
陈玲玲 中国医学科学院 北京协和医学院 药用植物研究所药理毒理中心,北京;湖南中医药大学,湖南长沙
孙秀萍 中国医学科学院 北京协和医学院 药用植物研究所药理毒理中心,北京
宋广青 中国医学科学院 北京协和医学院 药用植物研究所药理毒理中心,北京
王琼 中国医学科学院 北京协和医学院 药用植物研究所药理毒理中心,北京;中国航天员科研训练中心,北京
常琪 中国医学科学院 北京协和医学院 药用植物研究所药理毒理中心,北京
李海清 北京鑫海华仪科技有限公司,北京
李莹辉 中国航天员科研训练中心,北京
曲丽娜 中国航天员科研训练中心,北京
刘新民 中国医学科学院 北京协和医学院 药用植物研究所药理毒理中心,北京
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      ObjectiveTo apply reward directed operant conditioning task for learning and memory research in laboratory animals. Methods Sixteen adult male SHR rats and 8 Wistar rats were included in this study. Operant conditioning procedure was used to assess the learning ability of Wistar and SHR rats with magazine training, fixed ratio, signal discrimination and extinction procedure. ResultsSignificant difference was found in magazine training. The SHR rats performed more NP vs. Wistar rats and SHR rats treated with donepezil (P<0.05). There was no significant difference during FR1 schedule. While in higher response ratio FR2 and FR4 schedule, the SHR group showed significantly higher error responses (vs. Wistar rat group, P<0.05), lower rewards eared (vs. Donepezil-treated SHR rat group, P<0.001), and significantly lower correct accuracy rate (vs. Wistar rats and Donepezil-treated SHR rats, P<0.05). Significantly higher error responses were found during signal discrimination task (vs. Wistar rats and Donepezil-treated SHR rats, P <0.05), and lower correct accuracy rate (vs. Wistar rats and Donepezil-treated SHR rats, P<0.05). No significant difference was found during extinction. Neurotransmitter test showed that the hippocampal content of glutamate [(1.0639±0.07086) mg/g], acetylcholine [(2.7760±0.2609)μg/g] and 5-HT [(1.2200±0.1137)μg/g] were significantly lower in the SHR group (vs. Wistar rat group, P<0.05). The content of acetylcholine [(3.9344±0.2747)μg/g] was higher in the Donepezil-treated SHR rats group (vs. Wistar rats,P<0.05;vs. SHR rats, P<0.001). Conclusions Reward directed operant conditioning is a type of positive reinforced conditioning. This task can be used as a new behavior test method for learning and memory study.
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