Expression of Smad3 protein during the pulmonary fibrosis induced by paraquat in mice
  
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DOI:10.3969/j.issn.1005-4847.2012.01.019
KeyWord:Paraquat; Intoxication; Pulmonary fibrosis; Smad3; Immunohistochemistry; mice
           
AuthorInstitution
董雪松 中国医科大学附属第一医院急诊科,沈阳
刘伟 中国医科大学附属第一医院急诊科,沈阳
刘淑英 中国医科大学附属第一医院急诊科,沈阳
刘志 中国医科大学附属第一医院急诊科,沈阳
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Abstract:
      ObjectiveThe expression of Smad3 protein in the pulmonary fibrosis induced by paraquat (PQ) was detected by immunohistochemistry. Methods57BL/6J male mice(n = 58)were divided randomly into the experimental group and control group. Pulmonary fibrosis was induced by intraperitoneal injection of PQ(10 mg/kg)in the experimental group(n = 48), while physiological saline was used in the control group(n = 10)by the same method. The mice were sacrificed on days 2,5, 7 and 14 in the experimental group and the control ones on day 7. The lungs were taken for histological examinatioon using HE staining and the expression of Smad3 protein was determined by immunohistochemistry. ResultsThe histological changes including edema, hemorrhage and inflammatory cell infiltration were seen in the lung tissues of mice after PQ administration on days 2 and 5. There were slight collagen deposition and plaque-like fibrosis on day 7 and apparent fibrosis on day 14. The Smad3-positive signs appeared in the nucli of infiltrating macrophages and many type Ⅱ alveolar epithelial cells on days 2,5 and 7. The positive expression of Smad3 proteins was positively correlated with the amount of infiltrating macrophages. On day 14 the faint Smad3-positive expression was detected in the nuclei of fibroblasts in hyperplastic foci. ConclusionsThe expression of Smad3 protein is abnormal during pulmonary fibrosis induced by PQ and Smad3 plays an important role in the development of pulmonary fibrosis.
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