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| Cloning and eukaryotic expression of mouse Uncv gene |
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| DOI：10.3969/j.issn.1005-4847.2012.01.017 |
| KeyWord:Uncv; clone; eukaryotic expression; hair follicle |
| Author | Institution |
| 徐嫄 |
北京工商大学,北京 ;军事医学科学院实验动物中心, 北京 |
| 刘冰 |
军事医学科学院实验动物中心, 北京 |
| 李文龙 |
军事医学科学院实验动物中心, 北京 |
| 胡仲明 |
军事医学科学院实验动物中心, 北京 |
| 曾林 |
军事医学科学院实验动物中心, 北京 |
| 何聪芬 |
北京工商大学,北京 |
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| Abstract: |
| ObjectiveTo clone the full length cDNA of Uncv gene in mice and to express the gene in eukaryotic cells.MethodsRT-PCR assay was applied to clone the full length coding region of the Uncv gene and constructed its expression plasmid pcDNA 3.1-Flag/Uncv. The recombinant plasmid was transfected into HeLa cells and the fusion protein was identified by Western blot analysis.ResultsThe complete coding sequence was obtained and cloned into the pcDNA 3.1-Flag vector. The recombinant pcDNA 3.1-Flag/Uncv plasmid was transiently expressed in HeLa cells. HeLa cell clones expressing fusion protein with molecular weight of about 95×103 were obtained. ConclusionsA recombinant eukaryotic expression plasmid of Uncv has been successfully constructed and expressed in HeLa cells. It may provide a foundation for further biological studies of Uncv gene. |
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