Construction and identification of DNA libraries enriched for microsatellite repeat sequences of Chinese hamster
  
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KeyWord:Chinese hamster;Microsatellite;Enriched library;DNA;
SONG Gu-hua,GENG Jia-ning,JIA Ruo-yu,YUE Wen-bin,LIU Tian-fu,HU Song-nian
SONG Gu-hua1,2,GENG Jia-ning3,JIA Ruo-yu3,YUE Wen-bin2,LIU Tian-fu1,HU Song-nian3(1.Laboratory Animal Center,Shanxi Medical University,Taiyuan 030001,China,2.College of Animal Science and Technology,Shanxi Agricultural University,Taigu 030801,3.Key Laboratory of Genome Information and Sciences,Beijing Institute of Genomics,Chinese cademy of Sciences,Beijing 100029)
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Abstract:
      Objective To screen the microsatellite loci of Chinese hamster DNA to serve the genetic studies of germplasm resources,classification and evolution of Chinese hamsters.Methods Genomic DNAs from Chinese hamster was fragmented by ultrasonication.The fragments in size from 500 bp to 1000 bp were recovered by 2% agarose gel electrophoresis and ligated to SNX linkers with T4 DNA ligase,then denatured and hybridized to 14 biotinylated oligonucleotides.The biotinylated hybrids were retained on magnetic beads accor...
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