Isolation of microsatellite markers in tree shrews(Tupaia belangeri chinensis)
  
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DOI:
KeyWord:Tree shrew;Microsatellite;Molecular marker;
LI Jing-xiao,WANG Xin-xing,WANG Wen-guang,SUN Xiao-mei,DAI Jie-jie
LI Jing-xiao,WANG Xin-xing,WANG Wen-guang,SUN Xiao-mei,DAI Jie-jie(Institute of Medical Biology,Chinese Academy of Medical Science and Peking Union Medical College,Kunming 650118,China)
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Abstract:
      Objective To isolate microsatellite markers in tree shrews(Tupaia belangeri chinensis),and to fill the gap of lack of specific genetic markers of tree shrews.Methods A partial genomic library was constructed in tree shrews.Thirty-six positive clones were isolated from screening about 1500 clones of the genomic library with(CA)15 probe labelled at the 5'end with digoxin.Sequences of these clones and 15 microsatellites were isolated which included one repeated clones and one short flank sequence.Results The o...
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