Growth characteristics and transgenosis of bovine cumulus cells
  
View Full Text  View/Add Comment  Download reader
DOI:
KeyWord:Bovine;cumulus cells;Cell culture;Liposomes;Cell transfection;Transgene;
LU Zi-li,WANG Liang,SHI Guo-qing
LU Zi-li1,2,WANG Liang3,SHI Guo-qing4,1(1.College of Animal Science & Technology,Shihezi University,Shihezi 832000,China,2.Urumqi Maternal and Child Health Care Hospital,Urumqi 830001,3.Xinjiang Institute of Physics and Chemistry,Chinese Academy of Science,Urumqi 830011,4.Institute of Animal Husbandry and Veterinary,Xinjiang Agricultural Reclamation Academy,Shihezi 832000)
Hits: 387
Download times: 0
Abstract:
      Objective To understand the growth characteristics of bovine cumulus cells and the transfection efficiency of cumulus cells,based on the gene vector pGC1-collagen-EGFP-Neor that containing human collagen type I cDNA gene and mammary gland specific promoter,constructed in our laboratory.Methods Bovine cumulus cells were obtained by digestion of bovine cumulus/oocyte complexes(COCs) using hyaluronidase.Gene vector pMSCV-EGFP-Neor and pGC1-collagen-EGFP-Neor was transfected into cumulus cells with cationic lip...
Close
function PdfOpen(url){ var win="toolbar=no,location=no,directories=no,status=yes,menubar=yes,scrollbars=yes,resizable=yes"; window.open(url,"",win); } function openWin(url,w,h){ var win="toolbar=no,location=no,directories=no,status=no,menubar=no,scrollbars=yes,resizable=no,width=" + w + ",height=" + h; controlWindow=window.open(url,"",win); } &et=B070EDEAB21DF809BADB1CEC06A64CA53FC75FE1198C27AB5CFCD685C39634809AFF2FB060B7E89040E3AB6F73A57E4D6890EE3B1EAF26C3B935B3645B146BCB&pcid=&cid=&jid=&yid=9377ED8094509821&aid=99963042268&vid=&iid=E158A972A605785F&sid=&eid=&fileno=20110474&flag=1&is_more=0"> var my_pcid=""; var my_cid=""; var my_jid=""; var my_yid="9377ED8094509821"; var my_aid="99963042268";