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| Growth characteristics and transgenosis of bovine cumulus cells |
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| DOI： |
| KeyWord:Bovine;cumulus cells;Cell culture;Liposomes;Cell transfection;Transgene; |
| LU Zi-li,WANG Liang,SHI Guo-qing |
LU Zi-li1,2,WANG Liang3,SHI Guo-qing4,1(1.College of Animal Science & Technology,Shihezi University,Shihezi 832000,China,2.Urumqi Maternal and Child Health Care Hospital,Urumqi 830001,3.Xinjiang Institute of Physics and Chemistry,Chinese Academy of Science,Urumqi 830011,4.Institute of Animal Husbandry and Veterinary,Xinjiang Agricultural Reclamation Academy,Shihezi 832000)
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| Abstract: |
| Objective To understand the growth characteristics of bovine cumulus cells and the transfection efficiency of cumulus cells,based on the gene vector pGC1-collagen-EGFP-Neor that containing human collagen type I cDNA gene and mammary gland specific promoter,constructed in our laboratory.Methods Bovine cumulus cells were obtained by digestion of bovine cumulus/oocyte complexes(COCs) using hyaluronidase.Gene vector pMSCV-EGFP-Neor and pGC1-collagen-EGFP-Neor was transfected into cumulus cells with cationic lip... |
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