Abstract:Objective To investigate the effect of interleukin-4(IL-4) and interleukin-12(IL-12) on survival of purified retinal ganglion cells(RGCs),and to explore the association of autoimmune dysregulation with RGCs injury.Methods Mouse RGCs were isolated and purified using anti-mouse Thy1.2 monoclonal antibody(two steps of antibody adhesion).The purity of RGCs were verified by immunohistochemistry using double labeled Thy1.2 monoclonal antibody combined with microtubule-associated protein-2(MAP2) polyclonal antibod...