Effect of interleukin-4 and interleukin-12 on the survival of purified mouse retinal ganglion cells
  
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KeyWord:Retinal ganglion cell;Interleukin-4;Interleukin-12;Neuroprotection;Mouse;
WEI Wei,HUANG Ping,ZHANG Shao-dan,HUANG Chen,ZHANG Shao-min,ZHANG Chun,WANG Wei
WEI Wei 1,HUANG Ping1,ZHANG Shao-dan1,HUANG Chen1,2,ZHANG Shao-min3,ZHANG Chun1,WANG Wei1 (1.Department of Ophthalmology,2.Medical Research Center,Third Hospital of Peking University,Beijing 100191,China,3.Department of Neural and Behavioral Science,Penn State University College of Medicine,Hershey,PA 17033,USA)
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Abstract:
      Objective To investigate the effect of interleukin-4(IL-4) and interleukin-12(IL-12) on survival of purified retinal ganglion cells(RGCs),and to explore the association of autoimmune dysregulation with RGCs injury.Methods Mouse RGCs were isolated and purified using anti-mouse Thy1.2 monoclonal antibody(two steps of antibody adhesion).The purity of RGCs were verified by immunohistochemistry using double labeled Thy1.2 monoclonal antibody combined with microtubule-associated protein-2(MAP2) polyclonal antibod...
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