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| Human umbilical cord mesenchymal stem cells support prolonged undifferentiated growth of human embryonic stem cells |
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| DOI： |
| KeyWord:Human embryonic stem cells;Mouse embryonic fibroblast cells;Human umbilical cord mesenchymal stem cells;Feeder cells;Clinical application; |
| LIU Feng,XU Yong-sheng,YU Han-yan,WANG Wei,ZHANG Chun |
LIU Feng1,3,XU Yong-sheng2,YU Han-yan2,WANG Wei1,ZHANG Chun1,2,3(1.Department of Ophthalmology,2.Clinical Stem Cell Research Center,Peking University Third Hospital,Beijing 100191,China,3.Key Lab of Ministry of Education,Beijing 100191)
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| Abstract: |
| Objective To find a new source of human feeder cells which can support the undifferentiated growth of human embryonic stem cells.In this study,human umbilical cord mesenchymal stem cells(HUCMSCs) were tested as feeder cells for supporting the growth of hESCs to prevent the possibility of contamination from animal feeder cells.MethodsMesenchymal stem cells were isolated from human umbilical cord by collagenase IV digestion.Their surface markers were detected by flow cytometry.The cells were induced to differ... |
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