Abstract:Objective To investigate whether synaptic NMDA receptor activation induces Wnt non-canonical signaling pathway activation.Methods Various methods such as primary cortical neuron culture,immunofluorescence staining,double immunofluorescence staining,and Western blotting combined with NMDA activation were used to assess the increase of some proteins in Wnt non-canonical pathway.Results The methods of primary cortical neuron culture were established and the purity of neurons was enough for experiment.Wnt5a was...