Pressure perfusion with collagenase type Ⅱ improves the efficacy of isolation of adult rat cardiomyocytes
  
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KeyWord:Rat;Cardiomyocytes;cell separation;
LI De,TANG Bing,YANG Da-chun,MA Shuang-tao,YANG Yong-jian
LI De,TANG Bing,YANG Da-chun,MA Shuang-tao,YANG Yong-jian(Department of Cardiology,Chengdu Military Area Command General Hospital,Chengdu 610083,China)
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Abstract:
      Objective To explore a more effective method of isolation of adult rat cardiomyocytes.Methods Car-diomyocytes from SD rats were isolated by collagenase type II perfusion in the control group,and by pressure perfusion with collagenase type II in the experimental group.The morphology of cardiomyocytes was observed by optical microscopy and the cardiomyocyte viability was evaluated by trypan blue exclusion test and local field stimulation.Results The ratio of rod-shaped cells and yield of viable cells per rat ...
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