Vitrification of bovine oocytes affects their gene mRNA abundance
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Q95-33 R-114

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    Abstract:

    Objective To evaluate the mRNA expression abundance of genes,which are related with embryonic development and ability to undergo successful fertilization,cleavage and blastocyst development,in the GV stage and in vitro matured(IVM) bovine oocytes.Methods The oocytes that vitrified-warmed at GV(n = 224) and IVM(n = 235) were in vitro fertilized and cultured up to blastocyst stage under standard conditions.In parallel,we analyzed the relative a-bundance of Bax,Dnmt1,Hdac1,cyclinb1,Cdc2 and Cu Zn SOD transcrip...

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