Vitrification of bovine oocytes affects their gene mRNA abundance
  
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KeyWord:Vitrification;Gene expression;Expression abundance;Bovine oocyte;Developmental competence;
LIN Jia-peng,WANG Jing,ZHAO Yun-cheng,CHEN Tong,WANG Li-qin,CHEN Bo,HUANG Jun-cheng
LIN Jia-peng1,WANG Jing1,2,ZHAO Yun-cheng1,CHEN Tong1,WANG Li-qin1,CHEN Bo1,HUANG Jun-cheng1(1.Xinjiang Academy of Animal Sciences,The Key Laboratory of Livestock Reproduction & Breed Biotechnology of Ministry of Agriculture,Xinjiang Academy of Biotechnological Center,Urumqi 830000,China,2.Xinjiang Agricultural University Agronomy College,Urumqi 830052)
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Abstract:
      Objective To evaluate the mRNA expression abundance of genes,which are related with embryonic development and ability to undergo successful fertilization,cleavage and blastocyst development,in the GV stage and in vitro matured(IVM) bovine oocytes.Methods The oocytes that vitrified-warmed at GV(n = 224) and IVM(n = 235) were in vitro fertilized and cultured up to blastocyst stage under standard conditions.In parallel,we analyzed the relative a-bundance of Bax,Dnmt1,Hdac1,cyclinb1,Cdc2 and Cu Zn SOD transcrip...
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