Objective To establish a golden hamster model of hyperlipidemia.Methods 20 golden hamsters were randomly divided into control and experimental groups,10 per each group.The hamsters of experimental group were treated with high cholesterol diet for 15 days.During the experiment,the gross health status was observed.Finally,the contents of serum total cholesterol(TC),triglyceride(TG),high-density lipoprotein-cholesterol(HDL-C),low-density lipoprotein-cholesterol(LDL-C) and very low-density lipoprotein-cholesterol(VLDL-C) were assayed,and pathological changes of the liver were examined by light and electron microscopy.Results\ The golden hamsters showed to be well toleranble to high cholesterol diet.After 15 days,the contents of serum TC, TG,HDL-C,LDL-C and VLDL-C were 9.06,4.19,2.43,6.21,18.88 fold-increased,respectively(P<0.01),but the ratio of HDL-C/(LDL-C VLDL-C) decreased significantly compared with that in the normal group(P<0.05).The pathological examination exhibited liver structural alterations.Conclusion It is a quite rapid and reliable way to establish hyperlipidemia model of golden hamster,which can continuously provide enough plasma for hyperlipidemia research.