Determination of Genetic Purity of Bombyx mori Inbred Strain by RAPD
  Revised:October 14, 2004
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KeyWord:Bombyx mori,Inbred strain,Heredity,Random Amplified Polymorphic DNA Technique
TONG Xiao-ling,DAI Fang-yin ,YU Quan-you,YAN Hong,LU Cheng ,WEI Hong,XIANG Zhong-huai
TONG Xiao-ling1,DAI Fang-yin 1,YU Quan-you1,YAN Hong1,LU Cheng 1*,WEI Hong2,XIANG Zhong-huai1
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Abstract:
      Objective To analyze the genetic purity of silkworm (Bombyx mori) inbred strain IS-c108A for directing the breeding of silkworm as a laboratory animal. Methods 20 selected single primers were used to amplify randomly genomic DNA of 30 individuals in each batch of the inbred strain IS-c108A, parent strain c108 of this inbred strain and the control strain 871. The amplified fragment bands were analyzed to calculate the similarity index and the genetic distance among the individuals and batches. Results The frequency of polymorphic bands in the three batches of the inbred strain IS-c108A were 1.807%, 1.841% and 1.841%, respectively, and the average was 1.830%. The frequency of polymorphic bands was 7.207% between 30 individuals of the parent strain c108 of this inbred strain,while it was 7.08% between individuals of the control strain 871. The frequency of polymorphic bands between the inbred strain IS-c108A and c108 was 49.20%,while it was 58.33% between c108 and 871. Based on RAPD patterns, the calculated similarity index between 30 individuals in each batch of the inbred strain IS-c108A were 0.99581, 0.99555 and 0.99551, respectively, the average was 0.99562. Conclusion All the results implied that the inbred strain IS-c108A of silkworm has reached a high degree of genetic purity, and the silkworm has the advantage to obtain an inbred strain with high degree of purity easily.
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