Exploration on Relevant Indexes in Isoprenaline-induced Rat Myocardial Ischemia Models
  Revised:September 08, 2004
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KeyWord:Isoprenaline,Ischemia, myocardial,Model,Rats
JIA Guang Le ,DONG Pei Zhi
JIA Guang Le 1,DONG Pei Zhi 2
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Abstract:
      Objective To further discuss the indexes which can be used to objectively appraise the rat myocardial ischemia models. Methods To observe the effects of intraperitoneally injected isoprenaline in a dose of 10 mg/kg b.w. on the changes of electrocardiogram (ECG) and to assay the serum content of creatine kinase (CK), lactate dehydrogenase (LDH) and free fatty acids (FFA), and to measure the necrotic area of myocardia, the superoxide dismutase (SOD) and malondialdehyde (MDA) contents in cardiac muscle of Sprague_Dawley rats, compared with those of control rats received saline injection. Results The J point of ECG in myocardial ischemia model rats descented 30s after injection of isoprenaline (10 mg/kg) and recovered slowly after 2 min, but could not return to the original state within 20 minutes, and the J points at each time points within 20 min were significantly different from those in control rats ( P <0 05). T waves of ECG in myocardial ischemia model rats were also decreased since 30 s after s.c. injection of isoprenaline and were significantly different from those in control rats within 10 min. ( P <0 05). T waves of ECG in myocardial ischemia model rats at 30 s and 5 min after s.c. injection of isoprenaline were significantly different from those of control rats ( P <0 01 and P <0 05, respectively). The content of CK, LDH, FFA in serum and MDA in cardiac muscle of model rats were significantly higher than those of the control rats and the area of myocardial necrosis was positively correlated with those alterations. But SOD in cardiac muscle was negatively correlated with them. Conclusions The J point of ECG can be considered as a main index and T waves as an adjuvant index to evaluate this animal model, and the changes should be monitored within 20 min after drug administration. Some biochemical indexes such as CK, LDH, FFA, SOD or MDA are also helpful in studying the mechanisms and effects of anti_myocardial ischemia drugs.
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