Objective To establish standardized maintaining methods for mouse models of human diseases and long term conservation of their genetic characteristics. Methods\ Three strains of mice were selected as experimental objects: FVB/ TgN, MRL/ Mpj and SAMP1/ Ka. Comparing to specific controls, considerable differences were detected in growth curves, RAPD, isoenzyme electrophoresis and behavior tests and thus established a set of detecting methods to provide evidence of maintenance. Result\ The growth curves of MRL/Mpj mice differed statistically from those of controls. The RAPD diagrams of FVB/TgN, MRL/Mpj and SAMP1/Ka mice differed each other in their positive primers and bands and may provide as another item of characteristic indexes. The critical loci of isoenzyme can be indicated by isoenzyme electrophoresis. Moreover, behavior tests revealed in a more direct way multiple aspects of differences of these strains. Conclusion\ The study demonstrated that special measurements such as growth curves, RAPD, isoenzyme electrophoresis and behavior tests are needed in addition to routine examinations to determine specific characteristics of particular mouse strains in time, providing evidence of strain maintenance and avoid occurrence of genetic drift.