Objective To study the activities of the seroenzyme of the Smad3 gene knock|out mice.Methods The parameters of ALP,AST,ALT,CK,LDH|L of the 35 days,70 days and 6 months mice were measured by VITALAB made in Holland.Results The parameters of the homozygous mutation mice were the highest among that of the three gene types.ALP decreased with age,but AST,ALT,CK and LDH|L increased with age.Conclusion The activities of the seroenzymes changed due to lacking of the gene.