The Derivation and Examination of Embryonic Stem Cell Colonies in 615 Mouse
  Revised:February 08, 2002
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KeyWord:Mice, inbred strains,Cell line
ZHOU Xin,DONG Wanwei,WANG Taiyi
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Abstract:
      Objective To isolate ES cell colonies in 615 mouse. Method\ ES cell colonies in 615 mouse were isolated by PMEF feeder layer and the primary examination of these colonies without feeder layer was made. Results\ The formation rate of ES cell colonies and the rate of successful propagation were 22.6% and 0.94%,with positive ALP staining, stable karyotype and differentiation ability. Conclusion\ ES cell colonies in 615 mouse were isolated successfully.
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