Biological Characteristics Studies of an Established Model of Xenografted Human Ovarian Carcinoma in SCID Mice and Its Homologous Cell Lines in Vitro
  Revised:December 04, 2000
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KeyWord:Ovarian neoplasm, carcinoma,Mice, SCID,Neoplasm, experimental,Cell line
GE Hailiang,FONG Di,MA Anlun,ZHANG Huizhen,WANG Ying,YUAN Ming,CAI Xiaomin
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Abstract:
      Objective To establish a model of xenografted human ovarian carcinoma in severe combined immunodificiency (SCID) mice and homologous cell lines in vitro. Method A human ovarian serous papillary carcinoma derived from a surgical specimen was transplanted in the subcutis of SCID mice. After growth they were passaged mice to mice, meanwhile a human ovarian carcinoma cell isolated from xenografted tumor in mice was cultured in vitro and coloned for six months. The biological characteristics of xenografted tumor and homologous cells were determined by cell and molecular biological techniques. Results The xenografted tumor in SCID was successively passaged for 5 generations and a successful inoculation rate (90%) was observed during a period of 14 months. A tumor cell line (OVA 319) was established in 6 months and grown steadily, which isolated from the xenografted tumor. Histology and ultrastructure of microscopy showed that xenograted tumor retained its original growth characteristics and morphological properties. The chromosomal analysis of xenografted tumor cells and OVA 319 cells revealed aneuploid pattern of 12 46 in number, exhibiting features of human carcinoma. Furthermore, the FCM analysis and RT PCR technique showed same features as those of patient with original tumor tissue and xenografted tumor and OVA 319 cells, including distribution of cell cycle and it grew rapidly, and the expression of MAGE 2 gene at the mRNA level was observed. Conclusion Establishment of xenografted human ovarian carcinoma in SCID mice and OVA 319 cell lines could be considered as a model and provided experimental material for further investigation of human ovarian tumor.
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