|
| Isolation of ES Cell Colonies on a Feeder Layer of ICR Mouse Fibroblasts |
| |
| View Full Text View/Add Comment Download reader |
| DOI： |
| KeyWord:Mice,Fibroblasts,Embryonic stem cell |
| GAO Shuping,WANG Taiyi,SHI Weihong,DONG Wanwei |
|
| Hits: 817 |
| Download times: 0 |
| Abstract: |
| Objective To isolate ES cell colonies on a feeder layer. Methods Mouse embryos of 13-14 days were for the cultivation of primary mouse embryo fibroblasts (PMEF) which turned to be a feeder layer for blastocysts. Results The inner cell mass (ICM) of blastocysts grew well on the feeder layer of PMEF.The proliferation rate of ICM was 57%. After 48 hours of ICM disagregation ES colonies began to appear,showing strong AKP activity. Conclusion It is a preliminary success for the feeder layer to produce embryonic stem cell lines. |
| Close |
|
|
|