Study on Detection of Mouse Hepatitis Virus by Reserve Trascriptase and Polymerase Chain Reaction and Comparison of Different Primers
  
View Full Text  View/Add Comment  Download reader
DOI：
KeyWord:RT-PCR MHV IBV
Shao Wei-juan,Gao-Cheng,Lu Guo-pin,Bao Chun-yang,Wang Xiao-ming
Hits: 822
Download times: 0
Abstract:
      The Viral RNA extracted from cell infected with MHV1,MHV3,A59, JHM strains was transcribed into cDNA by using AMV reserse transcriptase, then t he cDNA was amplified by PCR. The MHV-specific fragments were obtained from all four strains with primer cpc1、2 and cpc3、4, but no band was oberserved in IBV standard and wild strains; A 241bp fragment was obtained from all four strains a nd IBV with primer cpc5、6. Virus RNA solutions were serially diluted and subjec ted to the RT-PCR analysis. The 10pg of viral RNA was detected.
Close
Copyright：ACTA LABORATORIUM ANIMALIS SCIENTIA SINICA
Address:5 Panjiayuan Nanli, Chaoyang District, Beijing 100021, China
E-mail: b67769337@126.com
Technical Support：Beijing E-tiller CO.,LTD.
function PdfOpen(url){ var win="toolbar=no,location=no,directories=no,status=yes,menubar=yes,scrollbars=yes,resizable=yes"; window.open(url,"",win); } function openWin(url,w,h){ var win="toolbar=no,location=no,directories=no,status=no,menubar=no,scrollbars=yes,resizable=no,width=" + w + ",height=" + h; controlWindow=window.open(url,"",win); } &et=08EE5A5866ACE44025BB42E6B80EBD21A2F8D3A9153AD429DF8E4C0988475AD8F7E5919538CCD5878BE761F92D89B20689BD5150BB5214200732C2A2518F1CACF0E2FF06BE23C3FD2B11F6C6FF14C24CD2D0B6D34DB5C166F8D0D8B87C2B2F1D69842EF24D560D2B485811A3DFBB31C528C38600860446E0B5A2843FB1FFFD9B8D57BEF794D81EDC&pcid=&cid=&jid=&yid=9806D0D4EAA9BED3&aid=3343586&vid=&iid=CA4FD0336C81A37A&sid=96C778EE049EE47D&eid=340AC2BF8E7AB4FD&fileno=20000109&flag=1&is_more=0"> var my_pcid=""; var my_cid=""; var my_jid=""; var my_yid="9806D0D4EAA9BED3"; var my_aid="3343586";