Extraction and Purification of Major Histocompatibility Complex Antigen in Mouse
  
View Full Text  View/Add Comment  Download reader
DOI:
KeyWord:Major Histocompatibility complex Detergent Affinity chromatography
Lan Ping,et al
Hits: 438
Download times: 0
Abstract:
      Objective:This study was designed to purify Major Histocompatibility Complex (MHC) antigen (H 2) that induce the rejection of organ transplantation in mouse.Methods:Triton/tris was used to extract the crude H 2,to which of the anti H 2 antibodies coupled with sepharose 4B was applied to purify H 2 antigen by affinity chromatography.The coupled H 2 Ag was eluted via 0.5%DOC,0.65M NaCl solution.Results:45kd heavy chain and 12kd light chain from purified proteins showed respectively by electrophoresis.Besides,the pure H 2 Ag was found to have serologically and biologically activity,as determined by antibodies rebinding and cytotoxicity inhibition assay.This method should be useful purification of a large quantity of H 2 Ag for the research in transplantation and functional analysis of MHC antigen.
Close
function PdfOpen(url){ var win="toolbar=no,location=no,directories=no,status=yes,menubar=yes,scrollbars=yes,resizable=yes"; window.open(url,"",win); } function openWin(url,w,h){ var win="toolbar=no,location=no,directories=no,status=no,menubar=no,scrollbars=yes,resizable=no,width=" + w + ",height=" + h; controlWindow=window.open(url,"",win); } &et=16DE616E3FFA863530ABBDAB210C3274402EFA00ABD8E84979F2866C613F0B500494D406B2DD34F90B402374C9F27A5CBDE946C853897BFD2A6CE9F92C7044428CE7117D4E4EE13A3A932FC31DD7421E595EECB2172CAA98&pcid=&cid=&jid=&yid=5370399DC954B911&aid=27119455&vid=&iid=CA4FD0336C81A37A&sid=&eid=&fileno=19970107&flag=1&is_more=0"> var my_pcid=""; var my_cid=""; var my_jid=""; var my_yid="5370399DC954B911"; var my_aid="27119455";