Abstract: To study the effect of auxiliary lipid-lowering function of preventive and therapeutic administration of Antarctic krill oil on experimental hyperlipidemic rats. Methods: We established hyperlipidemia model in SD rats that administrated of Antarctic krill oil for prevention and treatment respectively, then measured and analyzed the serum concentration of total cholesterol (TC), triglyceride (TG), high density lipoprotein cholesterol (HDL-C), low density lipoprotein cholesterol (LDL-C). Results: Antarctic krill oil preventive administration can significantly reduce serum TC, LDL-C(p＜0.05), which confirms with the results of liver pathology mutually, while no significant change of serum TG and HDL-C(p＞0.05). There was no significant statistical difference in serum concentration of TC, TG, HDL-C and LDL-C(p＞0.05) and no effect on auxiliary lipid-lowering after Antarctic krill oil therapeutic administration.Conclusion: There was significant effect on auxiliary lipid-lowering and reducing of TC after Antarctic krill oil preventive administration.