| 高占,高明周,高冬梅,王常瞵,乔明琦,高杰,王杰琼.基于数据挖掘的围绝经期抑郁症动物模型研究[J].中国实验动物学报,2022,30(7):887~896. |
| 基于数据挖掘的围绝经期抑郁症动物模型研究 |
| Animal model of perimenopausal depressive disorder based on data mining |
| 投稿时间：2022-04-13 |
| DOI：10. 3969 / j.issn.1005-4847. 2022. 07. 003 |
| 中文关键词: 围绝经期抑郁症 数据挖掘 卵巢摘除 慢性不可预见刺激 刺激因子 |
| 英文关键词:perimenopausal depressive disorder data mining ovariectomy chronic unforeseen stimuli stimulating factor |
| 基金项目: |
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| Author Name | Affiliation | | GAO Zhan | Chinese Medicine College of Shandong University of Traditional Chinese Medicine, Jinan 250355, China | | GAO Mingzhou | Institute of 'Traditional Chinese Medicine Imnovation，Shandong University of Traditional Chinese Medicine,Jinan 250355 | | GAO Dongmei | Chinese Medicine College of Shandong University of Traditional Chinese Medicine, Jinan 250355, China | | WANG Changlin | Chinese Medicine College of Shandong University of Traditional Chinese Medicine, Jinan 250355, China | | QIAO Mingqi | Lab of 'Traditional Chinese Medlicine Classical'Theory ，Ministry of Education，Shandong University of 'Traditional Chinese Medicine，Jinan 250355 | | GAO Jie | Chinese Medicine College of Shandong University of Traditional Chinese Medicine, Jinan 250355, China | | WANG Jieqiong | School of Pharmacy , Shandong University of Traditional Chinese Medicine, Jinan 250355 |
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| 中文摘要: |
| 目的 综述围绝经期抑郁症动物模型制备方法及评价指标,包括动物选择、卵巢摘除手术,CUMS刺激因子使用情况、造模和治疗起始时间选择、行为学检测等。 方法 收集CNKI和PubMed 、从建库到2022年5月期间关于围绝经期抑郁症动物模型的所有文献,依据纳入排除标准,筛选文献,提取相关信息,汇总信息、绘画图表。 结果 共纳入88篇文献,中文文献71篇,英文文献17篇;大鼠模型65篇,小鼠模型23篇。造模方法多采用“oVX+CUMS”和“OVX+CUMS+孤养”。大鼠(OVX)宜选择SD品系,90~120 d鼠龄,体重在200 g 以上;小鼠宜选择KM品系,20g左右;76%的研究选取7~8种刺激因子,每天随机1~2种且3~7 d内不重复出现,推荐选用禁食,禁水、夹尾,冰水游泳、昼夜颠倒;依据行为学结果进行分组的方式可减少实验鼠个体差异对实验的影响;阴道涂片一般开始于OVX手术后3 d,连续检测5 d;89%的研究选择“治疗与造模同时进行”或“造模结束后进行治疗”,造模与治疗时长宜保持一致,一般为2ld或28 d;阳性对照药多选取氟西汀或氟西汀结合雌激素;行为学实验多选用旷场实验、糖水偏好实验以及强迫游泳实验。 结论 本文筛选出应用最广泛的围绝经期抑郁症模型制备方法和评价指标,描述了造模过程的具体细节,有助于提高围绝经期抑郁症动物模型制备的可重复性。 |
| 英文摘要: |
| Objective To review the method used to prepare and evaluate animal models of perimenopausal depression, including animal selection, ovariectomy (OVX), chronic unpredictable mild stress (CUMS) stimulator use, modeling and treatment start time and behavioral testing. Methods We searched the CNKI and PubMed databases for studies of animal models of perimenopausal depression from the establishment of the databases to May 2022. Studies were screened according to defined inclusion and exclusion criteria, the relevant information was extracted and summarized, and charts were drawn. Results A total of 88 articles were identified, including 71 Chinese and 17 English articles. There were 65 rat models and 23 mouse models. The modeling method were OVX+CUMS and OVX+CUMS+solitary culture. Most studies in rats used SD rats (OVX), aged 90 ~ 120 days and weighing > 200 g; most studies in mice used KM mice, weighing about 20 g; 76% of the studies selected seven or eight stimulators, one or two of which were selected randomly each day and were not repeated within 3~ 7 days, with fasting, water deprivation, tail clipping, ice-water swimming, and day night reversal recommended method of simulation. Grouping according to behavioral result reduced the influence of individual differences. Vaginal smears were generally started 3 days after OVX and repeated for 5 days; 89% of the studies chose “treatment and modeling at the same time” or “ treatment after modeling”, and modeling and treatment should be consistent, generally 21 or 28 days. Positive control drugs included fluoxetine or fluoxetine combined with estrogen. The most common behavioral experiments were open field, sugar preference, and forced swimming experiments. Conclusions We reviewed the most widely used preparation method and evaluation indicators for animal models of perimenopausal depression, including details of the modeling processes, to improve the repeatability of preparing animal models of perimenopausal depression. |
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