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张百荣,李梦秋,范华娜,沈志滨,尹永芹,陶曙红,郑芳昊,陈艳芬.慢性皮肤溃疡大鼠模型的比较研究[J].中国实验动物学报,2022,30(3):392~399.
慢性皮肤溃疡大鼠模型的比较研究
Rat models of chronic skin ulcers: a comparative study
投稿时间:2021-10-12  
DOI:10. 3969 / j.issn.1005-4847. 2022. 03. 012
中文关键词:  慢性皮肤溃疡模型  大鼠  比较研究  糖皮质激素  金黄色葡萄球菌  异物
英文关键词:chronic skin ulcers model  rat  comparative study  glucocorticoid  staphylococcus aureus  foreign body
基金项目:
作者单位E-mail
张百荣 广东药科大学中药学院,广州 510006 1653481437@ qq. com 
李梦秋 广东药科大学中药学院,广州 510006 786514668@ qq. com 
范华娜 广东药科大学中药学院,广州 510006  
沈志滨 广东药科大学中药学院,广州 510006  
尹永芹 广东药科大学中药学院,广州 510006  
陶曙红 广东药科大学中药学院,广州 510006  
郑芳昊 佛山市中医院,广东 佛山 528000  
陈艳芬 广东药科大学中药学院,广州 510006 xwnai@ 163. com 
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中文摘要:
       目的 采用不同方法构建大鼠慢性皮肤溃疡模型,通过多种指标比较评价不同模型的差异和各自特征。 方法 大鼠随机分为 4 组,在建立全层皮肤缺损的开放性创面基础上施加不同因素,分别为:皮肤缺损组(皮损组)、皮肤缺损+激素干扰组(皮损+激素组)、皮肤缺损+激素干扰+菌感染组(皮损+激素+菌组)、皮肤缺损+异物组 (皮损+异物组);实验期间测量大鼠的体重,观察其活动状态及创面愈合情况,第 14 天取材,进行溃疡组织局部病理形态学观察,并检测免疫器官脏器指数、血液白细胞水平、肉芽组织中羟脯氨酸(Hyp)、总蛋白和肿瘤坏死因子-α (TNF-α)含量。 结果 实验期间各组大鼠体重没有显著性差异,造模后皮损组大鼠创面愈合较快,皮损+激素组的 “难愈”现象维持时间相对较短,皮损+异物组前期新生肉芽颜色灰白,但后期肉芽生长速度较快,而皮损+激素+菌组创面出现黄脓、肉芽组织生长速度较慢的特征;病理形态学观察显示,皮损+激素+菌组、皮损+异物组的炎症细胞浸润现象明显;与皮损组比较,其他 3 个模型组表现出脾指数增加、白细胞水平增加、创面组织羟脯氨酸含量显著降低(P< 0. 05 或 P< 0. 01),而且皮损+激素+菌组的总蛋白含量明显降低(P< 0. 05),TNF-α 含量明显增加(P< 0. 05)。 结论 在全层皮肤切除基础上叠加不同干预因素后,大鼠皮肤溃疡的愈合速度不同程度减慢,其中又以皮损+激素+细菌模型病理特征较稳定、持续时间长,可作为慢性难愈性皮肤溃疡的首选模型;皮损+激素、皮损+异物模型可能更适合短期难愈创面的有关研究,后者还可用于观察创面早期单纯肉芽生长情况的实验。
英文摘要:
       Objective To establish rat models of chronic skin ulcers by various methods and compare the differences and characteristics of the different models through various indexes. Methods Male SD rats were randomly divided into four groups and treated with various factors after creating a wound surface by removing back skin: skin defect, hydrocortisone (skin excision + hydrocortisone intervention), hydrocortisone plus bacterial interference (skin excision + hydrocortisone + bacterial intervention), and implantation of a foreign body (skin excision + embedded foreign body) groups. The body weight and wound healing of rats were measured each day and the wound state was observed. Materials were drawn from all groups at 14 days after the operation to assess histological changes, the immune organ index, blood leukocyte level, hydroxyproline (Hyp), total protein, and tumor necrosis factor-α (TNF-α) content in the skin ulcer. Results No significant difference in the body weight of rats in each group was found during the experimental period. The skin wounds of rats in the skin defect group healed faster, the symptom duration of chronic skin ulcers in the hydrocortisone group was shorter, and the color of granulation tissues in the implantation of the foreign body group was gray in the early stage, but the granulation grew faster in the later stage, while the hydrocortisone plus bacterial interference group had yellow pus in wounds and slow growth of granulation tissue. Pathomorphological observation showed severe inflammatory cell infiltration in hydrocortisone plus bacterial interference and implantation of foreign body groups. Moreover, compared with the skin defect group, the model groups showed increases in the spleen index, leukocyte level, and hydroxyproline content in wound tissue (P< 0.05 or P< 0.01), and in the skin lesion+ hormone +bacteria group, total protein content was decreased obviously (P< 0.05), while TNF-α content was increased significantly (P< 0.05). Conclusions After applying various intervention factors based on full- thickness skin resection, the healing speed of rat skin ulcers was slower than that of simple skin resection. Among them, the pathological characteristics of the skin lesion + hormone + bacteria model were more stable and long lasting. It can be used as the preferred model of chronic refractory skin ulcers, whereas the skin excision + hydrocortisone intervention and skin excision + foreign body embedded models may be more suitable to study short-term refractory wounds, and the embedded foreign body can be used to observe the growth of simple granulation in the early stage.
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