| 张源,武慧渊.小鼠室管膜细胞体外培养及多纤毛形成过程分析[J].中国实验动物学报,2022,30(3):370~375. |
| 小鼠室管膜细胞体外培养及多纤毛形成过程分析 |
| Primary culture of mouse ependymal cells and analysis of the multicilia formation process |
| 投稿时间：2021-12-22 |
| DOI：10. 3969 / j.issn.1005-4847. 2022. 03. 009 |
| 中文关键词: 室管膜细胞 动纤毛 小鼠 原代培养 |
| 英文关键词:ependymal cells multicilia mouse primary culture |
| 基金项目: |
|
|
| 摘要点击次数: 1056 |
| 全文下载次数: 355 |
| 中文摘要: |
| 目的 利用体外原代培养小鼠室管膜细胞(MEPC),表征其分化过程形态特征,纤毛的形成及摆动方式。 方法 通过将分离的新生小鼠端脑组织细胞进行特殊培养和分化,获得具有生理功能的 MEPC。 利用免疫组化方法跟踪 MEPC 分化过程中纤毛的形成,并在活细胞状态观察其纤毛摆动模式。 结果 分离的端脑细胞在特殊的培养条件下快速增殖,经过诱导分化可以得到具有多纤毛结构的 MEPC。 结论 本研究利用改进的原代 MEPC 培养方案,成功获得具有免疫组化特征及正常多纤毛摆动特性的 MEPC,并揭示了分化各个时期纤毛及相关 结构的形态特征,为以 MEPC 为模型的研究奠定了基础。 |
| 英文摘要: |
| Objective To use a modified protocol to obtain in vitro-cultured mouse ependymal cells (MEPCs) and to assess the morphological change of MEPCs during differentiation, the process of cilia formation, and the ciliary motility of mature MEPCs. Methods MEPCs with physiological functions were obtained by specialized culture and induced differentiation from isolated neonatal mouse telencephalon cells. Cilia formation and maturation of MEPCs were confirmed by immunocytochemistry. The ciliary beating pattern was recorded using a microscope equipped with a high-speed camera. Results The isolated telencephalon cells proliferated rapidly under culture conditions, and MEPCs with functional multicilia were obtained through induced differentiation. Conclusions Using a modified in vitro culture protocol, MEPCs with proper immunohistochemical characteristics and normal multicilia motility were successfully obtained. The process of multicilia formation at various stages of MEPC differentiation was revealed, providing a benchmark for further study using MEPCs as a model. |
| 查看全文 查看/发表评论 下载PDF阅读器 |
| 关闭 |
|
|
|
