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许林,魏颖,徐书静,张启云,郭静,周舒婷,孙黔云.中华眼镜蛇毒对大鼠凝血功能的影响[J].中国实验动物学报,2022,30(3):316~325.
中华眼镜蛇毒对大鼠凝血功能的影响
Effect of Naja atra venom on blood coagulation in rats
投稿时间:2021-10-25  
DOI:10. 3969 / j.issn.1005-4847. 2022. 03. 003
中文关键词:  中华眼镜蛇毒  凝血功能  蛇伤  血栓弹力图
英文关键词:Naja atra venom  blood coagulation function  snakebite  thromboelastogram
基金项目:
作者单位E-mail
许林 1. 贵州医科大学药学院,贵阳 550025
2. 贵州省中国科学院天然产物化学重点实验室,贵阳 550014 
xulin9712@ 163. com 
魏颖 1. 贵州医科大学药学院,贵阳 550025
2. 贵州省中国科学院天然产物化学重点实验室,贵阳 550014 
 
徐书静 贵州医科大学药学院,贵阳 550025  
张启云 贵州省中国科学院天然产物化学重点实验室,贵阳 550014  
郭静 2. 贵州省中国科学院天然产物化学重点实验室,贵阳 550014
3. 山西大学中医药现代研究中心,太原 030006 
 
周舒婷 贵州省中国科学院天然产物化学重点实验室,贵阳 550014  
孙黔云 1. 贵州医科大学药学院,贵阳 550025
2. 贵州省中国科学院天然产物化学重点实验室,贵阳 550014 
sunqy@ hotmail. com 
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中文摘要:
       目的 研究中华眼镜蛇毒对大鼠凝血功能的影响。 方法 体外实验采用中华眼镜蛇毒及其分离峰与大鼠全血孵育,测定血栓弹力图;体内采用尾静脉或肌肉注射眼镜蛇毒,取不同时间点全血测定血栓弹力图;取体内外实验分离的血浆测定 LDH 活力、血红蛋白、vWF、P-selectin 含量并采用酶切发色底物法测定凝血相关酶活性。 结果 体内外实验结果表明,眼镜蛇毒及其部分分离峰可明显引起大鼠凝血功能的异常,表现为凝血因子减少、纤维蛋白原水平低下、血小板功能减弱;同时大鼠血浆血红蛋白含量及 LDH 活力显著升高。 体外实验中眼镜蛇毒及其部分分离峰可显著下调酶切凝血酶及纤溶酶底物活性。 体内实验中肌肉注射后血浆中 P-selectin 含量 0. 5 h 时显著升高,酶切凝血因子Ⅹa 底物活性 6 h 内显著上调;尾静脉注射后血浆酶切凝血酶底物活性 2 h 内显著上调,6 h 内 vWF 含量明显升高。 结论 中华眼镜蛇毒能明显引起大鼠凝血功能的异常,其作用主要与激活凝血系统导致凝血因子消耗、纤维蛋白原减少以及血小板消耗等有关。
英文摘要:
       Objective To explore the effect of Naja atra venom on blood coagulation in rats. Methods Whole blood was incubated with Naja atra venom or various fractions in vitro and a thromboelastogram was obtained. In an in vivo experiment, Naja atra venom was injected via the tail vein or intramuscularly and whole blood samples were collected at various time points to obtain the thromboelastogram. LDH, hemoglobin, vWF, and P-selectin were measured in plasma. The activity of coagulation-related enzymes in plasma was measured by the chromogenic substrate method. Results The in vitro and in vivo result showed that Naja atra venom and some of its fractions obviously induced an abnormality in the coagulation function in rats, which was characterized by a lack of coagulation factors, low level of fibrinogen, and lowering of the platelet count and function, while the plasma hemoglobin content and LDH activity were increased significantly. In vitro, Naja atra venom and its fractions significantly decreased the enzymatic activities of cleaving S2238 and S2251. In vivo, after intramuscular injection, the content of P-selectin was significantly increased at 30 min and the enzymatic activity of cleaving S2765 was significantly increased within 6 h. After tail vein injection, the enzymatic activity of cleaving S2238 was increased significantly within 2 h and vWF content was increased obviously within 6 h. Conclusions Naja atra venom induces an obvious abnormality in the blood coagulation function of rats. Its effect is mainly related to the consumption of coagulation factors and fibrinogen caused by activation of the coagulation system and the consumption of platelets.
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