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何竹青,王利,王滢迪,郇鹏飞,杨玉芳,何建成.复方地黄颗粒对帕金森病阴虚动风证小鼠血清环核苷酸的调节及多巴胺能神经元的修复作用[J].中国实验动物学报,2022,30(3):309~315.
复方地黄颗粒对帕金森病阴虚动风证小鼠血清环核苷酸的调节及多巴胺能神经元的修复作用
Regulation of serum cyclic nucleotides and repair of dopaminergic neurons in mice with Yin deficiency and moving wind syndrome of Parkinson’s disease by compound Dihuang Granule
投稿时间:2021-10-14  
DOI:10. 3969 / j.issn.1005-4847. 2022. 03. 002
中文关键词:  阴虚动风证  帕金森病  病证结合  复方地黄颗粒  模型制备
英文关键词:Yin deficiency moving wind syndrome  Parkinson’ s disease  combination of disease and syndrome  Compound Dihuang Granules  model preparation
基金项目:
作者单位E-mail
何竹青 上海中医药大学,上海 201203 Zhuqing_He@ outlook. com 
王利 上海中医药大学,上海 201203  
王滢迪 上海中医药大学,上海 201203  
郇鹏飞 上海中医药大学,上海 201203  
杨玉芳 上海中医药大学,上海 201203 yyfapple@ icloud. com 
何建成 上海中医药大学,上海 201203 hejc8163@ 163. com 
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中文摘要:
       目的 探讨复方地黄颗粒对帕金森病(Parkinson’ s disease,PD) 阴虚动风证小鼠血清环核苷酸 (cAMP、cGMP)的调节及多巴胺神经元的修复作用。 方法 将 24 只雄性 C57BL/ 6 小鼠随机分为为正常组 (Normal)、模型组(MPTP+NS)、治疗组(MPTP+CDG),每组 8 只。 正常组小鼠注射生理盐水,模型组腹腔注射 1-甲基-4-苯基-1,2,3,6-四氢吡啶(1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine,MPTP)构建 PD 动物模型,两组均每日 1 次,连续注射 5 d。 治疗组造模后给予复方地黄颗粒(Compound Dihuang Granule,CDG)灌胃治疗,每日 1 次,连续给药 7 d。 本研究从 PD 小鼠病因、症状、客观指标检测、以方测证等方面验证该模型的中医证候属性,并探讨 CDG 对 PD 阴虚动风证小鼠血清中环核苷酸及酪氨羟化酶表达影响。 结果 模型组小鼠行为学、舌色度值比率、血清环核苷酸含量、酪氨酸羟化酶含量与正常组差异显著(P< 0.05)。 给予 CDG 治疗后能显著改善小鼠的行为学及相关客观指标(P< 0.05)。 结论 给予 MPTP 腹腔注射制备的帕金森病阴虚动风证小鼠模型稳定、可靠,CDG 能调节血清中环核苷酸含量,有效修复损伤的多巴胺能神经元。
英文摘要:
       Objective To investigate regulation of serum cyclic nucleotides (cAMP and cGMP) and the repair of dopamine neurons in mice with Yin deficiency and moving wind syndrome of Parkinson’ s disease ( PD) by Compound Dihuang Granule. Methods Overall, 24 male C57BL/ 6 mice were randomly divided into normal ( Normal), model (MPTP + NS), and treatment (MPTP + CDG) groups with eight mice in each group. The normal group was injected with normal saline and the model group was intraperitoneally injected with 1-methyl-4-phenyl-1,2,3,6-tetrahydropyridine (MPTP) to establish the PD animal model. Both groups were injected once a day for 5 consecutive days. The treatment group was administered Compound Dihuang Granule (CDG) by gavage once a day for 7 days. The TCM syndrome attribute of the model was verified by etiology, symptoms, the objective index, and formula test in PD mice and the effect of CDG on expression of cyclic nucleotides and tyrosine hydroxylase in the serum of PD mice with Yin deficiency and moving wind syndrome was assessed. Results Significant differences in behavior, the tongue chroma value ratio, serum cyclic nucleotide content, and tyrosine hydroxylase content were found between model and normal control groups (P< 0.05). CDG treatment significantly improved the behavior and related objective indexes of mice (P< 0.05). Conclusions The mouse model of Yin deficiency and moving wind syndrome of Parkinson’ s disease prepared by intraperitoneal injection of MPTP is stable and reliable. CDG regulates the content of cyclic nucleotides in serum and effectively repairs damaged dopaminergic neurons.
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