| 汤红日,王灵军,徐红玲,谢治梅,蔡娟,郑明辉,贺莉芳,刘晖.蝇类抗性品系培育的研究进展[J].中国实验动物学报,2022,30(1):124~130. |
| 蝇类抗性品系培育的研究进展 |
| Breeding for resistance strains in flies: a review |
| 投稿时间：2021-08-14 |
| DOI：10. 3969 / j.issn.1005-4847. 2022. 01. 016 |
| 中文关键词: 蝇 抗药性 抗性品系 培育 |
| 英文关键词:flies resistance resistance strains breeding |
| 基金项目: |
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| Author Name | Affiliation | E-mail | | TANG Hongri | Department of Parasitology, Zunyi Medical University, Zunyi 563003, China | hongritang1@ 163.com | | WANG Lingjun | Department of Parasitology, Zunyi Medical University, Zunyi 563003, China | | | XU Hongling | Department of Parasitology, Zunyi Medical University, Zunyi 563003, China | | | XIE Zhimei | Department of Parasitology, Zunyi Medical University, Zunyi 563003, China | | | CAI Juan | Department of Parasitology, Zunyi Medical University, Zunyi 563003, China | | | ZHENG Minghui | Department of Parasitology, Zunyi Medical University, Zunyi 563003, China | | | HE Lifang | Department of Parasitology, Zunyi Medical University, Zunyi 563003, China | | | LIU Hui | Department of Parasitology, Zunyi Medical University, Zunyi 563003, China | liuhui6032@ sina.com |
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| 中文摘要: |
| 随着杀虫剂的广泛应用,农业、卫生类病媒生物抗药性日益严重,不仅影响果蔬生产,危害人类健康,更带来环境污染和生态破坏等问题。 昆虫抗性品系培育作为研究抗药性机制和抗性治理的一种重要实验室研究手段,越来越受到研究者的关注。 蝇是一类分布广泛的公共卫生和农业害虫,通过抗性品系培育研究也可为有害蝇类的抗性机制研究和虫害防制提供可靠的实验数据和理论基础。 本文综述了蝇类等昆虫的生理生化抗性、行为抗性、遗传变异和共生菌抗性等抗药性机制,点滴法、浸渍法、药膜法、喷雾法等多种蝇类抗性品系的培育方法, 以及毒力测定、抗性倍数计算、基因表达、遗传变异等抗性品系的鉴定方法和影响因素,最终通过对比不同抗性品系培育和鉴定方法对蝇类抗药性研究的影响,以期为蝇类抗药性机制研究和抗药性监测与综合治理等提供参考。 |
| 英文摘要: |
| Insecticide resistance amongst agricultural pests and disease vectors are an increasing threat to human health, and the production of fruits and vegetables with the overuse of pesticides is causing serious ecological damage and environmental contamination. The breed of strains with resistance to insects has become an important laboratory approach in studies on resistance mechanisms and insect-pest control, and has gained growing attention from scientists worldwide. Research on breeding strains with resistance to flies, which are one of the widespread agricultural and public health pests, can also provide reliable data and the theoretical basis to understand the resistance mechanism of harmful flies and for future pest management. The present review performed a systematic summary on the following fields. First, we review the resistance mechanisms of flies and other insects, exploring physiological, biochemical, behavioral, genetic variation, and symbiotic resistance. Second, we review four cultivation method frequently applied for flies, such as topical application, dipping, residual film and spraying. Third, we summarize the identification method and influencing factors of resistant strains, such as virulence determination, resistance multiplier calculation, gene expression, and genetic variation. Finally, we compare the recent identification and breeding resistant varieties method . In conclusion , this review aims to provide significant application value and research insights for studies of resistance mechanisms, resistance monitoring and integrated pest management. |
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