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王诗雨,林家锋,蒋欣如,孙淼,王颖,陶晓莉.基于 16S rRNA 测序分析两种呼肠孤病毒对小鼠肠道菌群的影响[J].中国实验动物学报,2022,30(1):100~106.
基于 16S rRNA 测序分析两种呼肠孤病毒对小鼠肠道菌群的影响
Analysis of the effects of two reoviruses on the intestinal flora of mice based on 16S rRNA sequencing
投稿时间:2021-03-01  
DOI:10. 3969 / j.issn.1005-4847. 2022. 01. 013
中文关键词:  呼肠孤病毒  16S rRNA  肠道菌群  感染方式
英文关键词:reovirus  16S rRNA  intestinal flora  infection way
基金项目:
作者单位E-mail
王诗雨 锦州医科大学基础医学院病原生物学教研室,辽宁 锦州 121000 2692458551@ qq.com 
林家锋 锦州医科大学基础医学院病原生物学教研室,辽宁 锦州 121000 215470273@ qq.com 
蒋欣如 锦州医科大学基础医学院病原生物学教研室,辽宁 锦州 121000  
孙淼 锦州医科大学基础医学院病原生物学教研室,辽宁 锦州 121000  
王颖 锦州医科大学基础医学院病原生物学教研室,辽宁 锦州 121000  
陶晓莉 锦州医科大学基础医学院病原生物学教研室,辽宁 锦州 121000 taoxiaoli@ jzmu.edu.cn 
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中文摘要:
       目的 探究 T1L 和 NBV 两种呼肠孤病毒对小鼠肠道菌群结构的影响。 方法 将 25 只小鼠随机分为 5 组(对照组、NBV 滴鼻组、NBV 灌胃组、T1L 滴鼻组、T1L 灌胃组),每组 5 只。 对照组用 PBS 灌胃,其余组别均用 2 × 107 PFU/ mL 病毒滴度感染小鼠。 7 d 后采集小鼠粪便,从每组 5 个样本中选出粪便重量较重的 3 个样本,对粪便 DNA 进行 V3+V4 可变区特异性扩增,运用 16S rRNA 测序分析小鼠粪便中菌群的丰度、多样性及物种组成结构。 结果 T1L 和 NBV 灌毒后的小鼠肠道菌群丰度和多样性与对照组相比有所下降,且 T1L 滴鼻组下降最为显著 (P< 0. 05);与 NBV 灌胃组相比,NBV 滴鼻组的菌群丰度和多样性增加显著(P< 0. 05)。 在门的级别上,T1L 和 NBV 灌胃组中厚壁菌(Firmicutes)丰度明显减少,T1L 和 NBV 滴鼻组中拟杆菌(Bacteroidetes)丰度明显减少;在属的级别上,T1L 滴鼻组、T1L 灌胃组和 NBV 灌胃组中罗姆布茨菌(Romboutsia)丰度明显减少,T1L 滴鼻组中别样杆菌 (Alistipes)丰度明显增加(P<0. 05)。 结论 T1L 和 NBV 感染小鼠会降低菌群的丰度和多样性,可能通过有益菌减少或致病菌增多来破坏菌群平衡。 此外,病毒的感染方式不同,对菌群的影响也有所不同。
英文摘要:
       Objective To explore the effects of two reoviruses, T1L and NBV, on the structure of mouse intestinal flora. Methods The mice were randomly divided into five groups: control, instillation-NBV ( int-NBV), oral- NBV, instillation-T1L ( int-T1L ), and oral-T1L groups, with five mice in each group. The control group was intragastrically administered with phosphate buffered saline, and other groups were each infected with a viral titer of 2×107 PFU/ mL. After 7 days, mouse feces were collected and three samples were selected using the heavier stool weights from each group. After V3+V4 amplification, 16S rRNA technology was used to estimate the richness, diversity and composition of bacterial flora. Results After T1L and NBV instillation, the abundance and diversity of intestinal flora were lowered compared with the control group, with a significant decrease (P<0. 05) in the int-T1L group. The abundance and diversity were markedly augmented (P<0. 05) in the int-NBV group compared with the oral-NBV group. At the phylum level, the abundance of Firmicutes was markedly reduced in both the oral-T1L and oral-NBV groups compared with the control groups, and the abundance of Bacteroidetes was markedly reduced in both the int-T1L and int-NBV groups compared with the control group. At the genus level, Romboutsia was markedly lower in the int-T1L, oral-T1L and oral-NBV groups versus the control group, and Alistipes was markedly augmented in the int-T1L group (P< 0. 05). Conclusions Two reoviruses, T1L and NBV, reduced the abundance and diversity of mouse flora and may destroy flora balance through the reduction of beneficial bacteria or the increase of pathogenic bacteria. Different infection routes have different effects on the flora of mice.
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