壳聚糖对 PM2.5 所致小鼠急性肺损伤的干预作用
作者:
作者单位:

新乡医学院公共卫生学院,河南 新乡 453003

作者简介:

通讯作者:

中图分类号:

基金项目:


Intervention effect of chitosan on acute lung injury induced by PM2.5 in mice
Author:
Affiliation:

Department of Public Health Xinxiang Medical University, Xinxiang 453003, China

Fund Project:

  • 摘要
  • |
  • 图/表
  • |
  • 访问统计
  • |
  • 参考文献
  • |
  • 相似文献
  • |
  • 引证文献
  • |
  • 资源附件
  • |
  • 文章评论
    摘要:

    目的 探讨壳聚糖对 PM2.5所致小鼠急性肺损伤的干预作用。 方法 将 44 只雄性 C57BL/ 6J 小鼠随机分为 4 组,即对照组、PM2.5组、壳聚糖组及壳聚糖+PM2.5组。壳聚糖+PM2.5 、壳聚糖组提前 2 周灌胃壳聚糖,对照组和 PM2.5组灌胃蒸馏水,每天 1 次。 2 周后 PM2. 5暴露组气管滴注 PM2.5 ,对照组和壳聚糖组滴注生理盐水,每天 1 次,连续 7 d。 末次染毒 24 h 后处死动物。 HE 染色形态学观察,分光光度法测定肝丙二醛(MDA)、肺泡灌洗液 (BALF)中总蛋白(TP)和乳酸脱氢酶(LDH)水平;ELISA 法测定 BALF 和血清中白介素-1β(IL-1β)、白介素-8(IL- 8)及肿瘤坏死因子-α(TNF-α)表达。 结果 PM2.5组肺泡间隔显著增宽,其内有明显的淋巴、浆细胞浸润;相对于 PM2.5组,壳聚糖+PM2.5组肺间隔明显变窄,其内淋巴、浆细胞浸润明显减少。与对照组比较,PM2.5 组 MDA、TP、 LDH、TNF-α、IL-1β 和 IL-8 明显升高(P< 0. 05);与 PM2.5组比较,提前补充壳聚糖,MDA、TP、LDH、TNF-α、IL-1β 和 IL-8 明显降低(P< 0.05)。 结论 提前摄入一定剂量的壳聚糖,对 PM2.5所致肺损伤有一定的干预作用。

    Abstract:

    Objective To explore the intervention effect of chitosan on acute lung injury induced by particulate matter with a diameter ≤ 2.5 μm (PM2.5 ) in mice. Methods Forty-four specific pathogen-free male C57BL/ 6J mice were randomly divided into four groups: control, PM2.5 , chitosan and chitosan+PM2. 5 groups. Animals from the chitosan+ PM2.5 and chitosan groups were intragastrically administered chitosan daily for 2 weeks, and animals from the control and PM2.5 exposure groups were intragastrically administered distilled water once a day. After 2 weeks, the PM2.5 group was exposed to PM2.5 ( 4 mg / kg BW) via intratracheal instillation, whereas the control and chitosan groups were exposed to tracheal inhalation of normal saline, daily for 1 week. Animals were examined 24 h after the last exposure. Hematoxylin- eosin (HE) staining was used for morphological observation. The levels of malondialdehyde (MDA), total protein (TP) and lactate dehydrogenase ( LDH ) in alveolar lavage fluid ( BALF ) and liver samples were determined by spectrophotometry. The expression of interleukin-1β (IL-1β), interleukin-8 (IL-8) and tumor necrosis factor-α (TNF-α) in BALF and serum were determined by ELISA. Results HE staining showed that the alveolar septum was significantly widened in PM2.5 group, and there was obvious infiltration of lymphoid and plasma cells. The lung septum was markedly narrowed in the chitosan + PM2.5 group compared with the PM2.5 group, and infiltration of lymph and plasma cells was.

    参考文献
    相似文献
    引证文献
引用本文

熊程,赵英政,陶映君,徐光翠.壳聚糖对 PM2.5 所致小鼠急性肺损伤的干预作用[J].中国实验动物学报,2021,29(5):600~605.

复制
分享
文章指标
  • 点击次数:
  • 下载次数:
  • HTML阅读次数:
  • 引用次数:
历史
  • 收稿日期:2021-01-22
  • 最后修改日期:
  • 录用日期:
  • 在线发布日期: 2021-12-03
  • 出版日期:
征稿啦 |“科技伦理前沿谈”征文通知
关闭