| 韩子逸,沈诗怡,张金鑫,周燕,赵昕,刘明江,徐霄龙.角叉菜胶诱导的慢性血栓小鼠模型的建立[J].中国实验动物学报,2021,29(1):78~84. |
| 角叉菜胶诱导的慢性血栓小鼠模型的建立 |
| Preliminary establishment of chronic thrombus model in mice |
| 投稿时间：2020-06-16 |
| DOI：10. 3969 / j.issn.1005-4847. 2021. 01. 011 |
| 中文关键词: 慢性血栓 小鼠 角叉菜胶 氧化应激 炎性因子 |
| 英文关键词:chronic thrombosis mice carrageenan oxidative stress inflammatory cytokines |
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| 中文摘要: |
| 目的 采用不同浓度的角叉菜胶溶液连续腹腔注射的方式处理小鼠,筛选慢性血栓小鼠模型的条件。 方法 将 70 只 ICR 雄性小鼠随机分为 7 组,每组 10 只,即:空白组对照组、0. 02%剂量组、0. 04%剂量组、 0. 06%剂量组、0. 08%剂量组、0. 10%剂量组、0. 20%剂量组。 空白组小鼠腹腔注射生理盐水(0. 01 mL/ g),其余组腹腔注射相应浓度的角叉菜胶溶液(0. 01 mL/ g),每天注射 1 次;各组小鼠饲养于(20 ± 1)℃ 室温下并自由采食、 饮水。 每天观察各组出现黑尾的情况(黑尾率、黑尾长度);出栓即采样;采血检测血凝四项,检测结肠组织与血浆中氧化应激指标及炎性因子指标。 结果 0. 06%剂量组小鼠第 5 天出栓 2 只,第 6 天出栓 8 只;与空白对照组相比,0. 06%剂量组小鼠 FIB 含量显著升高(P< 0. 05),APTT 显著缩短(P< 0. 05),TT 显著延长(P< 0. 05),PT 极显著延长(P< 0. 01);0. 06%剂量组结肠组织与血浆中 SOD、GSH-Px 均极显著降低(P< 0. 01)、MDA 显著升高(P< 0. 05);0. 06%剂量组结肠组织与血浆中 TNF-α、IL-1β 显著升高(P< 0. 05)、IL-10 显著降低(P< 0. 05)。 结论 初步得出室温为(20 ± 1)℃条件下,0. 06%的角叉菜胶溶液(0. 01 mL/ g)连续腹腔注射 6 d(每天 1 次)是建立慢性 血栓小鼠模型的最适条件。 |
| 英文摘要: |
| Objective To screen the conditions of a chronic thrombus mouse model after injecting a carrageenan solution at various concentrations intraperitoneally. Methods Seventy ICR male mice were randomly divided into seven groups with 10 mice in each group: blank ( control), 0. 02%, 0. 04%, 0. 06%, 0. 08%, 0. 10%, and 0. 20% groups. Mice in the blank group were intraperitoneally injected with normal saline (0. 01 mL/ g), whereas mice in the other groups were intraperitoneally injected with carrageenan solutions at the corresponding concentration (0. 01 mL/ g) once a day. Mice in each group kept at (20 ± 1)°C and had free access to food and water. The black-tailed conditions (black-tailed rate and length) of each group were observed each day. An outlet plug was used for sampling. Blood samples were collected to detect blood clots, and indicators of oxidative stress and inflammatory factors in colon tissues and plasma were detected. Results In the 0. 06% dose group, two mice were thrombotic on 5 d and eight mice were thrombotic on day 6. Compared with the blank group, FIB content in the 0. 06% dose group was increased significantly ( P< 0.05), APTT was shortened significantly (P< 0.05), TT was prolonged significantly (P< 0.05), and PT was prolonged significantly (P< 0.01). SOD and GSH-Px in colon tissue and plasma of the 0. 06% dose group were decreased significantly (P< 0.01), and MDA was increased significantly (P< 0. 05). The colon tissue and plasma levels of TNF-α and IL-1β in the 0. 06% dose group were increased significantly (P< 0.05), and those of IL-10 were decreased significantly (P< 0.05). Conclusions A 0. 06% carrageenan solution (0.01 mL/ g) injected intraperitoneally for 6 d ( once a day) at (20 ± 1)°C is the optimal condition to establish a mouse model of chronic thrombosis. |
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