首页期刊介绍编委会投稿指南期刊订阅广告合作留言板联系我们English
韩子逸,沈诗怡,张金鑫,周燕,赵昕,刘明江,徐霄龙.角叉菜胶诱导的慢性血栓小鼠模型的建立[J].中国实验动物学报,2021,29(1):78~84.
角叉菜胶诱导的慢性血栓小鼠模型的建立
Preliminary establishment of chronic thrombus model in mice
投稿时间:2020-06-16  
DOI:10. 3969 / j.issn.1005-4847. 2021. 01. 011
中文关键词:  慢性血栓  小鼠  角叉菜胶  氧化应激  炎性因子
英文关键词:chronic thrombosis  mice  carrageenan  oxidative stress  inflammatory cytokines
基金项目:
作者单位E-mail
韩子逸 扬州大学兽医学院,江苏 扬州 225009 18151965089@ 163.com 
沈诗怡 扬州大学兽医学院,江苏 扬州 225009 1978640441@ qq.com 
张金鑫 扬州大学兽医学院,江苏 扬州 225009  
周燕 扬州大学兽医学院,江苏 扬州 225009  
赵昕 扬州大学兽医学院,江苏 扬州 225009  
刘明江 1.扬州大学兽医学院,江苏 扬州 225009
2.江苏省动物重要疫病与人畜共患病防控协同创新中心, 江苏 扬州 225009 
mjliu1@ 163.com 
徐霄龙 首都医科大学附属北京中医医院,北京 100010 xiaolong_xu3013@ 126.com 
摘要点击次数: 290
全文下载次数: 68
中文摘要:
       目的 采用不同浓度的角叉菜胶溶液连续腹腔注射的方式处理小鼠,筛选慢性血栓小鼠模型的条件。 方法 将 70 只 ICR 雄性小鼠随机分为 7 组,每组 10 只,即:空白组对照组、0. 02%剂量组、0. 04%剂量组、 0. 06%剂量组、0. 08%剂量组、0. 10%剂量组、0. 20%剂量组。 空白组小鼠腹腔注射生理盐水(0. 01 mL/ g),其余组腹腔注射相应浓度的角叉菜胶溶液(0. 01 mL/ g),每天注射 1 次;各组小鼠饲养于(20 ± 1)℃ 室温下并自由采食、 饮水。 每天观察各组出现黑尾的情况(黑尾率、黑尾长度);出栓即采样;采血检测血凝四项,检测结肠组织与血浆中氧化应激指标及炎性因子指标。 结果 0. 06%剂量组小鼠第 5 天出栓 2 只,第 6 天出栓 8 只;与空白对照组相比,0. 06%剂量组小鼠 FIB 含量显著升高(P< 0. 05),APTT 显著缩短(P< 0. 05),TT 显著延长(P< 0. 05),PT 极显著延长(P< 0. 01);0. 06%剂量组结肠组织与血浆中 SOD、GSH-Px 均极显著降低(P< 0. 01)、MDA 显著升高(P< 0. 05);0. 06%剂量组结肠组织与血浆中 TNF-α、IL-1β 显著升高(P< 0. 05)、IL-10 显著降低(P< 0. 05)。 结论 初步得出室温为(20 ± 1)℃条件下,0. 06%的角叉菜胶溶液(0. 01 mL/ g)连续腹腔注射 6 d(每天 1 次)是建立慢性 血栓小鼠模型的最适条件。
英文摘要:
       Objective To screen the conditions of a chronic thrombus mouse model after injecting a carrageenan solution at various concentrations intraperitoneally. Methods Seventy ICR male mice were randomly divided into seven groups with 10 mice in each group: blank ( control), 0. 02%, 0. 04%, 0. 06%, 0. 08%, 0. 10%, and 0. 20% groups. Mice in the blank group were intraperitoneally injected with normal saline (0. 01 mL/ g), whereas mice in the other groups were intraperitoneally injected with carrageenan solutions at the corresponding concentration (0. 01 mL/ g) once a day. Mice in each group kept at (20 ± 1)°C and had free access to food and water. The black-tailed conditions (black-tailed rate and length) of each group were observed each day. An outlet plug was used for sampling. Blood samples were collected to detect blood clots, and indicators of oxidative stress and inflammatory factors in colon tissues and plasma were detected. Results In the 0. 06% dose group, two mice were thrombotic on 5 d and eight mice were thrombotic on day 6. Compared with the blank group, FIB content in the 0. 06% dose group was increased significantly ( P< 0.05), APTT was shortened significantly (P< 0.05), TT was prolonged significantly (P< 0.05), and PT was prolonged significantly (P< 0.01). SOD and GSH-Px in colon tissue and plasma of the 0. 06% dose group were decreased significantly (P< 0.01), and MDA was increased significantly (P< 0. 05). The colon tissue and plasma levels of TNF-α and IL-1β in the 0. 06% dose group were increased significantly (P< 0.05), and those of IL-10 were decreased significantly (P< 0.05). Conclusions A 0. 06% carrageenan solution (0.01 mL/ g) injected intraperitoneally for 6 d ( once a day) at (20 ± 1)°C is the optimal condition to establish a mouse model of chronic thrombosis.
查看全文  查看/发表评论  下载PDF阅读器
关闭
您是第 2013874 位访问者
版权所有:中国实验动物学会 主管单位:中国科学技术协会 主办单位:中国实验动物学会    中国医学科学院医学实验动物研究所
地  址: 北京市朝阳区潘家园南里5号 邮编:100021 电话:010-67779337 E-mail:bjb2@cnilas.org
本系统由北京勤云科技发展有限公司设计
微信关注二维码