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胡倩倩,李炳,佘曼,李涛,周晓东.多巴胺 D1 受体在频闪光诱导性近视发生发展中作用的研究[J].中国实验动物学报,2021,29(1):71~77.
多巴胺 D1 受体在频闪光诱导性近视发生发展中作用的研究
Preliminary study on the effects of dopamine D1 receptor on the development of flickering light-induced myopia in guinea pigs
投稿时间:2020-09-02  
DOI:10. 3969 / j.issn.1005-4847. 2021. 01. 010
中文关键词:  近视  多巴胺 D1 类受体  频闪光诱导  SCH 23390
英文关键词:myopia  dopamine D1-like receptor(D1DR)  flickering light-induction  SCH 23390
基金项目:
作者单位E-mail
胡倩倩 复旦大学附属金山医院,上海 201508 18211270007@ fudan.edu.cn 
李炳 复旦大学附属金山医院,上海 201508  
佘曼 复旦大学附属金山医院,上海 201508  
李涛 复旦大学附属金山医院,上海 201508  
周晓东 复旦大学附属金山医院,上海 201508 xdzhou2013@ 162.com 
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中文摘要:
       目的 研究多巴胺 D1 类受体(D1DR)在频闪光诱导性近视(flickering light-induced myopia, FLM)发生中的作用,初步探讨 FLM 的发病机制。 方法 36 只 2 周龄豚鼠随机分成 4 组(n= 9):对照组、频闪光(FLM)组、 频闪光+溶剂(FLM + Vehicle)组、频闪光+D1DR 拮抗剂(FLM + SCH 23390)组。 分别于造模前后测量各组豚鼠的屈光度和眼轴长度,造模 6 周后采用免疫组化和免疫印迹方法检测视网膜中 D1DR 的表达变化,采用高效液相色谱电化学检测法(HPLC-ECD)测定视网膜中多巴胺(DA)及其代谢产物(DOPAC)的含量。 结果 与对照组相比, 频闪光组屈光度和眼轴长度呈显著的近视样改变(P< 0. 001,P< 0. 05),视网膜 D1DR 的表达明显升高(P< 0. 05), DA 含量显著上升以及 DOPAC/ DA 比值显著下降(P< 0. 001);而玻璃体腔注射 SCH 23390 可显著改善 FLM 豚鼠 屈光度和眼轴的变化(P< 0. 001,P< 0. 05),使 FLM 豚鼠视网膜 DA 含量下降(P< 0. 001)和 DOPAC/ DA 比值增加 (P< 0. 05)。 结论 D1DR 参与了 FLM 的形成,D1DR 拮抗剂可能通过抑制 D1DR 并影响 DA 及其代谢水平而改善 FLM 豚鼠的近视样改变。
英文摘要:
       Objective To study the effects of dopamine D1-like receptor ( D1DR) on the development of flickering light-induced myopia ( FLM) in guinea pigs and preliminarily explore the potential pathogenesis of FLM. Methods Thirty-six 2-week-old guinea pigs were randomly assigned to four groups ( n= 9): Control, FLM, FLM + Vehicle, and FLM+D1DR antagonist ( SCH 23390) groups. The refraction and axial length (AL) were measured before and after treatments. Expression of retinal D1DR was detected by immunohistochemistry and western blotting after 6 weeks of treatment. High performance liquid chromatography with electrochemical detection was used to detect the levels of DA and its primary metabolite (DOPAC) in the retina. Results Compared with those in the Control group, guinea pigs in the FLM group showed a significant myopic refractive shift and AL elongation (P< 0. 001), higher retinal D1DR expression (P< 0. 05), higher retinal DA levels, and a lower retinal DOPAC/ DA ratio (P< 0. 001). However, the increases of myopia (P< 0. 001) and AL (P< 0. 05) were significantly smaller in guinea pigs of the FLM+SCH 23390 group than in those of the FLM group. Additionally, lower retinal DA levels (P< 0. 001) and a higher retinal DOPAC/ DA ratio (P< 0. 05) were found in the FLM + SCH 23390 group than in the FLM group. Conclusions D1DR is involved in the development of FLM in guinea pigs. Blocking D1DR with SCH 23390 may suppress the development of FLM in guinea pigs, which might play an important role by influencing retinal DA levels and its metabolism.
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