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徐松涛,李嘉颖,沈利叶,郁晨,徐雁云,潘永明,孙志超,陈民利.超声、高分辨率磁共振成像技术在诊断评估兔颈动脉粥样硬化中的应有研究[J].中国实验动物学报,2021,29(1):9~16.
超声、高分辨率磁共振成像技术在诊断评估兔颈动脉粥样硬化中的应有研究
Application of ultrasound and high-resolution magnetic resonance imaging in the diagnosis and evaluation of carotid atherosclerosis in rabbits
投稿时间:2020-09-16  
DOI:10. 3969 / j.issn.1005-4847. 2021. 01. 002
中文关键词:  颈动脉粥样硬化  超声  高分辨率磁共振成像  动物模型  
英文关键词:carotid atherosclerosis  ultrasound  high-resolution magnetic resonance imaging  animal model  rabbit Conflicts of Interest: The authors declare no conflict of interest.
基金项目:
作者单位E-mail
徐松涛 浙江中医药大学动物实验研究中心/ 比较医学研究所,国家中医药管理局实验动物三级实验室,杭州 310053 xst0412@ 163.com 
李嘉颖 浙江中医药大学附属第一医院,医学影像科,杭州 310006  
沈利叶 浙江中医药大学动物实验研究中心/ 比较医学研究所,国家中医药管理局实验动物三级实验室,杭州 310053  
郁晨 浙江中医药大学动物实验研究中心/ 比较医学研究所,国家中医药管理局实验动物三级实验室,杭州 310053  
徐雁云 浙江中医药大学动物实验研究中心/ 比较医学研究所,国家中医药管理局实验动物三级实验室,杭州 310053  
潘永明 浙江中医药大学动物实验研究中心/ 比较医学研究所,国家中医药管理局实验动物三级实验室,杭州 310053  
孙志超 浙江中医药大学附属第一医院,医学影像科,杭州 310006  
陈民利 浙江中医药大学动物实验研究中心/ 比较医学研究所,国家中医药管理局实验动物三级实验室,杭州 310053 cmli991@ zcmu.edu.cn 
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中文摘要:
       目的 利用超声、高分辨率磁共振成像(HRMRI)技术,分析评价兔颈动脉粥样硬化(AS)模型的病变程度,为颈动脉 AS 动物模型的诊断评估提供技术方法。 方法 取雄性日本大耳白兔 18 只,随机分为正常对照 (NC)组和模型(Model)组,每组 9 只,模型组饲喂高胆固醇饲料 2 周后行右侧颈动脉球囊损伤术,术后继续高脂饲喂 6 周,建立兔颈动脉 AS 模型,NC 组饲喂常规饲料至 8 周。 造模 4 周、8 周时检测血浆总胆固醇(TC)和甘油三酯 (TG)并超声检查颈动脉,造模 8 周时行颈动脉高分辨率磁共振成像(HRMRI)检查,行安死术后取右侧颈总动脉行 HE 染色和油红“O”染色,评估颈动脉 AS 病变。 结果 与 NC 组比,模型组兔造模 4 周时血浆 TC 显著升高(P< 0. 05),8 周时体重、血浆 TC、TG 均显著增加(P< 0. 05,P< 0. 01);超声结果显示,模型组兔的右侧颈总动脉内中膜厚度(IMT)、狭窄率、收缩期最高血流速度、血管阻力指数均随病变程度加深而显著增加(P< 0. 05,P< 0. 01),并可见狭窄和斑块形成;HRMRI 结果亦显示,MIP 成像可见右侧颈总动脉明显狭窄,3D-TOF 成像可见模型组兔有明显 的 AS 斑块且狭窄率显著增加(P< 0. 01);组织病理学证实模型组兔颈总动脉有明显的 AS 斑块形成,IMT、狭窄率、 脂质含量均比 NC 组显著增加(P< 0. 01);相关性分析结果表明超声、HRMRI 与 HE 染色的定量分析结果显著相关 (P< 0. 01)。 结论 超声和 HRMRI 技术均能无创检测诊断兔颈动脉粥样硬化模型的病变,但 HRMRI 更能清晰、直观地判断血管狭窄及 AS 斑块严重程度。
英文摘要:
       Objective The aim of this study was to evaluate the application of ultrasound and high-resolution magnetic resonance imaging (HRMRI) in a rabbit carotid atherosclerosis (AS) model, providing technical method for the diagnosis and evaluation of preclinical atherosclerotic plaques. Methods Eighteen male Japanese white rabbits were randomly divided into two groups (n= 9). The model group was fed a high-cholesterol diet for 2 weeks followed by balloon injury to the right carotid artery. After surgery, high-fat feeding was continued for 6 weeks to establish a rabbit carotid AS model. The NC group was fed regular feed for 8 weeks. At 4 and 8 weeks after modeling, plasma total cholesterol (TC) and triglyceride ( TG) levels were measured, and the carotid artery was examined via ultrasound. Carotid HRMRI was performed at 8 weeks. After euthanasia, the right common carotid artery was taken for HE and oil red “O” staining to assess carotid artery atherosclerotic lesions. Results Compared with the findings in the NC group, TC levels were significantly higher in the model group at 4 weeks after modeling, and body weight and TC and TG levels were significantly higher in the model group at 8 weeks. The result of ultrasound revealed that intima-media thickness ( IMT), the stenosis rate, the maximum systolic blood flow velocity, and the vascular resistance index of the right common carotid artery increased significantly in the model group with the deepening of the lesions, and stenosis and plaque formation were observed. In addition, the HIMRI result disclosed obvious stenosis of the right common carotid artery compared with findings in the NC group, and the model group displayed obvious atherosclerotic plaque formation and a significantly higher stenosis rate. Histopathology further confirmed the obvious atherosclerotic plaque formation in the common carotid artery in the model group, and IMT, the stenosis rate, and lipid content were significantly higher in the model group than in the NC group. In addition, correlation analysis demonstrated that the quantitative result of ultrasound, HRMRI, and HE staining were significantly correlated. Conclusions Both ultrasound and HRMRI technology can non-invasively detect and diagnose lesions in a rabbit carotid AS model, but HRMRI can more clearly and intuitively judge the severity of vascular stenosis and atherosclerotic plaques.
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