| 楚璐萌,田子颖,崔蕊,吴娇,于海川.低氧对斑马鱼胚胎发育和红细胞生成的抑制作用[J].中国实验动物学报,2021,29(1):1~8. |
| 低氧对斑马鱼胚胎发育和红细胞生成的抑制作用 |
| Inhibition effects of hypoxia on embryonic development and erythropoiesis in zebrafish |
| 投稿时间：2020-05-27 |
| DOI：10. 3969 / j.issn.1005-4847. 2021. 01. 001 |
| 中文关键词: 斑马鱼 低氧 胚胎发育 造血分化 红细胞生成 |
| 英文关键词:zebrafish hypoxia embryonic development hematopoietic differentiation erythropoiesis |
| 基金项目: |
| 作者 | 单位 | E-mail | | 楚璐萌 | 1.新乡医学院医学检验学院,河南省分子诊断与医学检验技术协同创新中心,河南 新乡 453003
4. 河南省郑州市第七人民医院,郑州 450000 | 670381639@ qq.com | | 田子颖 | 新乡医学院医学检验学院,河南省分子诊断与医学检验技术协同创新中心,河南 新乡 453003 | | | 崔蕊 | 新乡医学院医学检验学院,河南省分子诊断与医学检验技术协同创新中心,河南 新乡 453003 | | | 吴娇 | 新乡医学院药学院,河南 新乡 453003 | wujiao@ xxmu.edu.cn | | 于海川 | 1.新乡医学院医学检验学院,河南省分子诊断与医学检验技术协同创新中心,河南 新乡 453003
3. 新乡医学院第二附属医院,河南省生物精神病学重点实验室, 河南 新乡 453002 | haichuan_yu@ 163.com |
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| 中文摘要: |
| 目的 本文以斑马鱼(Danio rerio)为研究对象,探讨了低氧对早期胚胎发育、造血分化和红系分化的影响。 方法 选取受精后 12 h 的斑马鱼胚胎,随机分为两组,以常氧组为对照组,低氧组为实验组,实时观察斑马鱼胚胎发育形态学的变化;通过联苯胺染色、邻联茴香胺染色、AO 染色及瑞氏吉姆萨染色观察红细胞的生成及形态学变化;并通过 Real time PCR 检测了斑马鱼胚胎造血相关基因的表达情况。 结果 与常氧相比,低氧降低了斑马鱼胚胎卵黄囊的营养消耗,抑制了色素细胞的形成,减慢了心率,延缓了斑马鱼胚胎的孵化,观察和分析了低氧对红细胞产生和成熟的抑制作用。 结论 低氧延缓了斑马鱼胚胎发育,抑制了红细胞的产生和成熟。 |
| 英文摘要: |
| Objective The vertebrate model of zebrafish (Danio rerio) was employed to explore the effects of hypoxia on early embryonic development, hematopoietic differentiation, and erythroid differentiation. Methods At 12h post-fertilization, zebrafish embryos were randomly divided into two groups. The normoxic group was used as the control group, and the hypoxic group was used as the experimental group. The morphological changes of zebrafish embryos were observed in real-time. Erythropoiesis and morphological changes were observed by benzidine, O-dianisidine, acridine orange, and May-Grunwald Giemsa staining. Real time PCR was used to analyze hematopoietic gene expression in zebrafish embryos. Results Hypoxia reduced nutritional consumption of the yolk sac, inhibited the formation of pigment cells, slowed down the heart rate, and delayed the hatching of zebrafish embryos. Inhibitive effects of hypoxia on the production and maturity of red blood cells were observed. Conclusions Hypoxia delays zebrafish embryonic development and inhibits the production and maturity of red blood cells. |
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