| 赵楠楠,王艳,郭艳娟,高杰,袁金灵,陈燕.Oct4 和 Cdx2 对牛分胚化胎的内影细响胞团和滋养外胚层[J].中国实验动物学报,2020,28(6):818~823. |
| Oct4 和 Cdx2 对牛分胚化胎的内影细响胞团和滋养外胚层 |
| Effects of Oct4 and Cdx2 on differentiation of bovine embryonic inner cell mass and trophectoderm |
| 投稿时间：2020-08-12 |
| DOI：10. 3969 / j.issn.1005-4847. 2020. 06. 012 |
| 中文关键词: Oct4 Cdx2 牛 胚胎发育 细胞分化 |
| 英文关键词:Oct4 Cdx2 bovine embryonic development cell differentiation |
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| 中文摘要: |
| 目的 研究 Oct4 和 Cdx2 敲低对牛 ICM 和 TE 分化相关的基因表达的影响,解释 Oct4 和 Cdx2 在牛胚胎早期发育中的作用。 方法 RNA 干扰敲低牛胚胎 Oct4 或 Cdx2 基因。 实时荧光定量 PCR 及免疫荧光染色检 测敲低 Oct4 或 Cdx2 基因对牛胚胎早期发育及相关基因表达的影响。 结果 针对 Oct4 或 Cdx2 基因的特异性 siRNA 能够抑制牛胚胎 Oct4 或 Cdx2 表达。 Oct4 敲低对胚胎卵裂率和 8-细胞率无影响,但胚胎囊胚率显著降低, mRNA 水平显著下降(P< 0. 05)。 Cdx2 敲低抑制 Oct4 mRNA 表达(P< 0. 05)。 结论 Oct4 通过调节 Cdx2、Sox2 及 Nanog 的表达控制牛胚胎的分化。 |
| 英文摘要: |
| Objective To investigate the effects of Oct4 and Cdx2 knockdown on the expression of genes involved in ICM (Inner Cell Mass) and TE (Trophoectoderm) differentiation in cattle, and to elucidate the roles of Oct4 and Cdx2 in the early development of bovine embryos. Methods Oct4 and Cdx2 genes were knocked down in bovine embryos by RNA interference. Real-time quantitative PCR and immunofluorescence staining were used to observe the effects of knockdown on early embryo development and gene expression. Results Specific siRNAs against Oct4 and Cdx2 inhibited Oct4 and Cdx2 expression, respectively, in bovine embryos. Oct4 knockdown had no effect on embryonic cleavage and eight-cell proportions, although the blastocyst proportion was significantly reduced; moreover, the mRNA levels of Cdx2, Sox2, and Nanog were significantly reduced (P < 0. 05). Cdx2 knockdown inhibited the expression of Oct4 mRNA (P < 0. 05). Conclusions Oct4 controls the differentiation of bovine embryos by regulating the mRNA expression levels of Cdx2, Sox2, and Nanog. |
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