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彭亮,杨鹏,刘冲,杨金丰,马三辉.钙和维生素 D 膳食补充对大鼠踝关节骨折创伤模型破骨细胞活性和骨量的影响分析[J].中国实验动物学报,2020,28(6):812~817.
钙和维生素 D 膳食补充对大鼠踝关节骨折创伤模型破骨细胞活性和骨量的影响分析
Effects of dietary calcium and vitamin D supplementation on osteoclast activity and bone mass in a rat ankle fracture model
投稿时间:2020-08-04  
DOI:10. 3969 / j.issn.1005-4847. 2020. 06. 011
中文关键词:    维生素 D  破骨细胞活性  骨量  踝关节骨折创伤模型
英文关键词:Ca  vitamin D  osteoclast activity  bone mass  trauma model of ankle fracture
基金项目:
作者单位E-mail
彭亮 定州市人民医院骨科,河北 定州 073000 45515287@ qq.com 
杨鹏 定州市人民医院骨科,河北 定州 073000  
刘冲 定州市人民医院骨科,河北 定州 073000  
杨金丰 定州市人民医院骨科,河北 定州 073000  
马三辉 定州市人民医院骨科,河北 定州 073000 y00jingf@ 163.com 
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中文摘要:
       目的 探讨钙和维生素 D(Ca / VitD)膳食对大鼠踝关节骨折创伤模型破骨细胞活性和骨量的影响。 方法 选择 8 周龄雄性 SD 大鼠为研究对象,将大鼠随机分为三组对照组(C)、Ca / VitD 缺乏饮食组(D)及 Ca / VitD 补充饮食组(S),建立踝关节骨折创伤模型。通过大鼠血清分析、微型计算机断层扫描(μCT)分析、组织形态计量学和免疫组化分析及大鼠创伤愈伤组织的基因表达分析研究 Ca / VitD 缺乏饮食是否会损害骨修复。此外还探讨了从创伤的时间点开始在饮食中补充 Ca / VitD 是否能增强骨折愈合。 结果 与 C 组相比,D 组骨密度(BMD)显著降低(P< 0. 05),骨量减少(P< 0. 001),纤维组织量增加(P< 0. 05)。 在骨折的愈伤组织中,D 组破骨细胞的数量和表面明显增加( P < 0. 01)。踝关节骨折后补充 Ca / VitD,与 D 组相比, S 组愈伤组织中的骨量显著增加 (P< 0. 001),而纤维组织减少(P< 0. 05)。此外,S 组表现出较高的骨折愈合率(P< 0. 05)。 S 组 I 型胶原 C 端端 肽(CTX)水平减少,碱性磷酸酶(ALP) 和 X 连锁磷酸盐调节基因( Phex) 表达增加(P< 0. 05)。与此同时,S 组 iFGF23 及 cFGF23 的血清水平均显著升高,与 D 组,C 组相比较具有显著统计学差异(P< 0. 05)。但 iFGF23: cFGF23 三组无显著统计学差异性(P> 0. 05)。 结论 手足创伤后补充 Ca / VitD 可减少 Ca / VitD 缺乏饮食者破骨细胞活性和骨量增加,改善骨修复。对临床术后护理具有指导意义。
英文摘要:
       Objective To investigate the effects of calcium and vitamin D ( Ca / VitD) supplementation on osteoclast activity and bone mass after hand and foot trauma. Methods Eight-week-old male mice were randomly divided into three groups: control ( C), Ca / VitD deficiency ( D), and Ca / VitD supplementary diet groups ( S). Group S underwent hand and foot trauma surgery after 8 weeks of standard feeding. Mouse serum analysis, micro-computed tomography (CT), histoeconometric analysis, immunohistochemical analysis, and gene expression analysis of fracture calli were used to investigate whether Ca / VitD deficiency could impair bone repair and cause bone loss after injury. The study also investigated whether Ca / VitD supplementation in the diet from the time of fracture could enhance fracture healing. Results Compared with the findings in group C, BMD and bone mass were significantly decreased in group D, whereas the fibrous tissue volume was increased. In the fracture callus, the number and surface of osteoclasts in group D were significantly enhanced. Compared with the result in group D, the bone mass in the callus was significantly increased in group S, whereas the amount of fibrous tissue was significantly decreased after Ca / VitD supplementation. In addition, group S exhibited a higher fracture healing rate. In group S, expression of the C-terminal telopeptide of type I collagen was decreased, whereas that of alkaline phosphatase and X-linked phosphate regulatory gene was increased. Meanwhile, the serum levels of iFGF23 and cFGF23 were significantly higher in group S than in groups D and C. However, the iFGF23: cFGF23 ratio was not different among the groups. Conclusions Ca / VitD supplementation after hand and foot trauma reduced osteoclast activity, increased bone mass, and suppressed bone absorption in mice with Ca / VitD deficiency. These findings should have guiding significance for clinical postoperative nursing.
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