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刘倩,鲁华,郭更新,刘静,李娜.连续性血液透析滤过联合依那普利对急性重症胰腺炎大鼠肾功能及肠功能的改善作用[J].中国实验动物学报,2020,28(6):805~811.
连续性血液透析滤过联合依那普利对急性重症胰腺炎大鼠肾功能及肠功能的改善作用
Continuous hemodiafiltration combined with enalapril improves renal and intestinal function in rats with severe acute pancreatitis
投稿时间:2020-06-01  
DOI:10. 3969 / j.issn.1005-4847. 2020. 06. 010
中文关键词:  连续性血液透析滤过  依那普利  急性重症胰腺炎  肾功能  肠功能
英文关键词:continuous hemodiafiltration  enalapril  severe acute pancreatitis  renal function  intestinal function Conflicts of Interest: The authors declare no conflict of interest.
基金项目:
作者单位E-mail
刘倩 邢台市人民医院肾内科,河北 邢台 054001 maozoua@ 163.com 
鲁华 邢台市人民医院肾内科,河北 邢台 054001  
郭更新 邢台市人民医院肾内科,河北 邢台 054001  
刘静 邢台市人民医院肾内科,河北 邢台 054001  
李娜 邢台市人民医院产科,河北 邢台 054001  
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中文摘要:
       目的 探究连续性血液透析滤过联合依那普利对急性重症胰腺炎大鼠肾功能及肠功能的改善作用。 方法 60 只 SD 大鼠随机分为 5 组:对照组、模型组、连续性血液透析滤过组、依那普利组及连续性血液透析滤过联合依那普利组,除对照组外均采用牛黄胆酸钠诱导急性重症胰腺炎大鼠模型,连续性血液透析滤过组造模后给予连续性血液透析滤过 12 h;依那普利组术前 7 d 灌胃 10 mg / (kg·d)的依那普利,每天 1 次;连续性血液透析滤过联合依那普利组作相应的联合处理。采用 HE 染色观察大鼠胰腺、肾及肠粘膜组织病理变化,检测血浆内毒素 (ET)、D-乳酸(D-LC)、血清淀粉酶(AMY)、尿素氮(BUN)和肌酐(Cr)水平,FITC-Dextran 通透实验检测肠道通透性,Western Blot 检测大鼠 α-血管平滑肌肌动蛋白(α-SMA)、E 钙黏素(E-cadherin)、高迁移率族蛋白 B1(HMGB1) 及闭合蛋白(occludin)的表达。 结果 与模型组相比,连续性血液透析滤过联合依那普利可有效减轻急性重症胰腺炎大鼠胰腺、肾及肠粘膜组织损伤,显著降低大鼠肠粘膜通透性、血清 AMY、BUN、Cr、血浆 ET、D-LC 水平、组织 α-SMA 及 HMGB1 的表达,显著提高 E-cadherin 和 Occludin 的表达,且作用效果优于单项处理。 结论 连续性血液透析滤过联合依那普利可明显改善急性重症胰腺炎所致的肾及肠组织损伤,有助于肾功能及肠功能的恢复。
英文摘要:
       Objective To explore the beneficial effects of continuous hemodiafiltration (CHDF) combined with enalapril on renal and intestinal function in rats with severe acute pancreatitis ( SAP). Methods Sixty Sprague-Dawley rats were randomly divided into a control group and four treatment groups: SAP model, CHDF, enalapril, and CHDF + enalapril. Rats in the treatment groups were induced with SAP using sodium taurocholate. After modeling, the CHDF group was given CHDF for 12 h, and the enalapril group was given enalapril 10 mg / kg / d by gavage, for 1 day. The CHDF + enalapril group was given both treatments simultaneously. Histopathological changes in the pancreas, kidney and intestinal mucosa were observed by hematoxylin and eosin staining. The plasma levels of endotoxin (ET) and D-lactic acid (D-LC), and serum levels of amylase (AMY), blood urea nitrogen ( BUN) and creatinine ( Cr) were measured. A fluorescein isothiocyanate-dextran permeability test was used to examine intestinal permeability. Western blotting was used to detect expression of α-vascular smooth muscle actin (α-SMA), E-cadherin, high mobility groupbox protein 1 ( HMGB1) and occludin. Results Compared with the model, CHDF + enalapril had the following effects in rats with SAP: effectively alleviated damage to the pancreas, kidney and intestinal mucosal tissues; significantly reduced intestinal mucosal permeability, the levels of serum AMY, BUN and Cr and plasma ET and D-LC, and expression of α-SMA and HMGB1 in tissues; and significantly increased expression of E-cadherin and occludin. The combined action effect was better than that of either single treatment. Conclusions CHDF combined with enalapril significantly improved renal and intestinal tissue damage caused by SAP, which contributed to the recovery of renal and intestinal function.
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