Objective To observe the expression levels of inflammatory factors, AP-1, and MCP-1 in TNF-α- stimulated vascular endothelial cells and co-cultured with smooth muscle cells following treatment with the compound Qishao Jiangya tablet, and explore the mechanism by which the compound Qishao Jiangya tablet inhibits vascular remodeling. Methods A co-culture system was established using vascular endothelial cells and smooth muscle cells. Stimulation with TNF-α was used to mimic the hypertensive inflammatory environment. The cultured cells were randomly divided into seven groups: normal control, model, blank serum control, MRS2578, irbesartan tablet drug-containing serum, compound Qishao Jiangya tablet-containing serum, and MRS2578 + compound Qishao Jiangya tablet-containing serum. Except the normal control group, all groups were stimulated with 10 ng / mL TNF-α; the irbesartan tablet drug-containing serum, compound Qishao Jiangya tablet-containing serum, and MRS2578+compound Qishao Jiangya tablet-containing serum were treated with 10% drug-containing serum; the MRS2578 and MRS2578+compound Qishao Jiangya tablet drug-containing serum groups were treated with 10 μm P2Y6 receptor blocker MRS2578. After 24 hours of modeling and drug intervention, CCK8 assays were used to detect the survival rates of endothelial cells and smooth muscle cells. ELISA was used to detect the level of IL-8 in the culture supernatant. Immunofluorescence, Western Blot, and quantitative PCR were used to detect endothelial expression levels of AP-1 and MCP-1. Results Compared with the normal control group, the survival rates of smooth muscle and endothelial cells decreased significantly in the model and blank serum control groups, while the levels of IL-8 were significantly higher (P < 0. 01). Compared with the normal control group,AP-1 and MCP-1 in endothelial cells in the model and blank serum control groups were detected by fluorescence intensity value of immunofluorescence, gray value ratio by Western Blot, and mRNA levels by quantitative PCR were significantly increased (P< 0. 01). Compared with the model group, the survival rates of smooth muscle and endothelial cells were significantly increased in the compound Qishao Jiangya tablet-containing serum groups, while the levels of IL-8 were significantly lower (P < 0. 05). AP-1 and MCP-1 in endothelial cells were detected by fluorescence intensity value of immunofluorescence, gray value ratio by Western blotting, and mRNA levels by quantitative PCR in the compound Qishao Jiangya tablet-containing serum groups were significantly reduced compared with the model group(P < 0. 01). Conclusions Following stimulation with TNF-α, the compound Qishao Jiangya tablet can improve the survival rates of endothelial cells and smooth muscle cells, reduce the levels of AP-1 and MCP-1, prevent endothelial cell inflammation, improve endothelial damage, and protect normal function of smooth muscle cells.