Animal experiments are essential for medical research, which has led to the sacrifice of many laboratory animals (LA). However, various emerging techniques can be used to replace experiments requiring LA sacrifice or products of LA sacrifice. In many instances, these techniques provide more reproducibility and parameter control than experiments involving LA or LA products, and in particular can reduce the sacrifice of LA and avoid issues with ethical approval during the experiment. In silico simulation, informatics, 3D cell culture models, new types of cell culture medium, and products of animal-friendly affinity reagents are some innovative technologies that can reduce the sacrifice of LA, reduce the cost of experiments, and ensure the accuracy of experimental result . At the same time, owing to species differences between animals and humans, the reliability of some experimental result related to human health has also been questioned. Finally, alternatives to animal experiments help to reduce the number of animals required for research to a certain extent, but cannot completely eliminate the need for animals in research. This article summarizes the progress of related alternative technologies in recent years, provides ideas for the development of relevant experiments in medical research, achieves the purpose of reducing LA sacrifice, and improves the welfare of LA.