兽用疫苗制造检验用 C 型产气荚膜梭菌菌种的 制备与检定标准和方法研究
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1.中国兽医药品监察所,北京 100081; 2.中国农业大学动物医学院,北京 100193

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Standard and method of preparation and verification of Clostridium perfringens (C) for production and testing of veterinary vaccines
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1.China Institute of Veterinary Drug Control, Beijing 100081, China. 2.College of Veterinary Medicine, China Agricultural University, Beijing 100193

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    摘要:

    目的 为制备兽用疫苗制造和检验用产气荚膜梭菌 CVCC60101 菌种,检定其生物学特性,完善其 制备、检定的标准和操作方法。 方法 对 1960 年冻干保存的 C 型产气荚膜梭菌菌株进行复苏、易感动物复壮、分离培养、分装冻干制备,对制备的菌种形态、培养、生化、血清学、毒力和免疫原性等生物学特性进行检定。 结果 制备的菌种纯粹,形态、生化和培养特性符合产气荚膜梭菌特征,真空度、剩余水分、血清学、毒力和免疫符合兽用疫苗制造和检验用菌种要求。 结论 本研究为完善 C 型产气荚膜梭菌菌种的制备、检定标准和操作方法提供了技术基础。

    Abstract:

    Objective To prepare Clostridium perfringens (CVCC60101) for production and testing of veterinary vaccines, verify its biological characteristics, and perfect the standard and method of preparation and verification. Methods In this study, Clostridium perfringens (C) prepared and stored by freeze-drying in 1960 was resuscitated, rejuvenated using susceptible animals, isolated, cultured, freeze-dried, and the new strain’s morphology, culture characteristics, biochemical properties, serological characteristics, virulence, immunogenicity, and other characteristics were tested. Results The result showed that the new strain was pure, and its morphology, culture characteristics, and biochemical properties accorded with the characteristics of Clostridium perfringens. In addition, its vacuum degree, residual moisture, serological characteristics, virulence, and immunogenicity accorded with the standard of the strain for production and testing of veterinary vaccines. Conclusions This research gives more experimental data for perfecting the standard and method of preparation and verification of Clostridium perfringens (C).

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彭国瑞,李旭妮,王秀丽,李伟杰,魏津,马欣,张一帜,田野,刘博,李建,罗玉峰,李俊平,彭小兵.兽用疫苗制造检验用 C 型产气荚膜梭菌菌种的 制备与检定标准和方法研究[J].中国实验动物学报,2020,28(5):627~631.

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  • 收稿日期:2019-06-01
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  • 在线发布日期: 2020-11-25
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