Objective To observe the therapeutic effects of electroacupuncture (EA) on mechanical pain of a CRPS-I rat model. To study the intervention of EA on the oxidative stress response from local tissues of a CRPS-I rat model and further investigate the mechanisms underlying acupuncture analgesia. Methods The chronic post-ischemic pain model (CPIP) was established to mimic CRPS-I. Healthy male SD rats were randomly divided into Sham, CPIP, CPIP + EA, and CPIP + Sham EA groups. After model establishment, EA was applied on a daily basis. The 50% paw withdrawal thresholds (PWTs) were measured by the von Frey hair test. Detection kits were used to measure the oxidative stress- related markers. Immunostaining was used to detect cellular oxidative damage. Results 1) Compared with those in the Sham group, the 50% PWTs were significantly decreased in CPIP group rats (P< 0. 01) and lasted until the end of the experiment, indicating the successful establishment of the CPIP rat model. 2) After EA treatment, the 50% PWTs were significantly increased in the CPIP + EA group ( P< 0. 01), compared with those in the CPIP + Sham EA group. 3) Compared with the Sham group, the CPIP group showed significantly decreased levels of SOD and GSH and increased levels of H2O2 , MDA, and 8-OHG in ipsilateral hind paw tissues (P< 0. 05 or P< 0. 01). 4) After EA treatment, compared with the CPIP + Sham EA group, the CPIP + EA group showed significantly increased levels of SOD and GSH and decreased levels of H2O2 , MDA, and 8-OHG (P< 0. 05 or P< 0. 01). Conclusions Our result indicate that EA can significantly reduce the oxidative stress response in ipsilateral hind paw tissues of CPIP model rats. This mechanism may be involved in EA’s therapeutic effect on mechanical pain of CPIP model rats.