首页期刊介绍编委会投稿指南期刊订阅广告合作留言板联系我们English
崔玮,丁玲强,曾巧英.甘青虎耳草乙酸乙酯提取物对小鼠肝癌细胞及原位移植瘤的抑制作用[J].中国实验动物学报,2020,28(4):463~469.
甘青虎耳草乙酸乙酯提取物对小鼠肝癌细胞及原位移植瘤的抑制作用
Inhibitory effect of EtOAc extract from Saxifraga taugutica on hepatocellular carcinoma cells and orthotopic-transplanted tumors in mice
投稿时间:2020-02-20  
DOI:10. 3969 / j.issn.1005-4847. 2020. 04. 005
中文关键词:  甘青虎耳草  乙酸乙酯提取物  肝癌细胞  原位移植瘤  小鼠
英文关键词:Saxifraga taugutica  EtOAc extract  hepatoma cells  orthotopic transplantation tumor  mice
基金项目:
作者单位E-mail
崔玮 1.河西学院生命科学与工程学院,甘肃 张掖 734000
2. 河西学院实验动物中心,甘肃 张掖 734000 
zhangyecw@ 163.com 
丁玲强 1.河西学院生命科学与工程学院,甘肃 张掖 734000
2. 河西学院实验动物中心,甘肃 张掖 734000 
 
曾巧英 甘肃农业大学动物医学院, 兰州 730070 zengqy@ gsau.edu.cn 
摘要点击次数: 138
全文下载次数: 44
中文摘要:
      目的 探讨甘青虎耳草乙酸乙酯提取物(EtOAc extract from Saxifraga taugutica,EST)对小鼠肝癌细胞及原位移植瘤的抑制作用。 方法 体外实验用不同浓度 EST 处理 H22 细胞 48 h 后,AO/ EB 双荧光染色,激光 共聚焦显微镜观察细胞形态变化;用核酸电泳和流式细胞术检测 H22 细胞的凋亡情况。 建立小鼠肝癌原位移植瘤 模型进行体内实验,对比 EST 低、中、高剂量组对荷瘤小鼠抑瘤率、T 淋巴细胞增殖能力以及肝病理变化的影响。 结果 EST 处理后 H22 细胞出现凋亡形态学变化,且与浓度正相关;核酸电泳呈凋亡特征性梯状带;流式细胞分析,500 μg / mL 处理组凋亡率达 31%;体内试验显示, EST 低、中、高剂量的抑瘤率分别为 33. 0%、46. 7%、64. 3%;显 著提高小鼠的胸腺指数、T 淋巴细胞增殖能力(P< 0. 05);组织学观察证明 EST 各剂量组癌细胞有不同程度的生 长抑制,形态固缩、向周围组织浸润减少。 结论 EST 可增强小鼠免疫功能和诱导肿瘤细胞凋亡,具明显的抗肿瘤作用,与剂量正相关。
英文摘要:
      Objective To explore the effect of EtOAc extract from Saxifraga taugutica (EST) on carcinoma cells and orthotopic-transplanted tumors. Methods Murine cancer cells ( H22 cells ) were incubated with different concentrations of EST for 48 h in vitro. Morphological changes were examined by laser confocal microscopy after AO/ EB double staining. Apoptosis of carcinoma cells was determined by DNA electrophoresis. and flow cytometry. Orthotopic- transplanted tumors in mice were established in vivo. The tumor inhibition rate, thymus index, spleen index, and liver pathology were compared between low-dose, medium-dose, and high-dose groups. Results Apoptotic morphological changes of H22 cells were observed, with the level of apoptosis gradually increasing with increased EST concentration. DNA electrophoresis demonstrated a typical ladder pattern. Flow cytometry revealed that the apoptosis rate of H22 cells was 31% in the EST group with 500 μg / mL. In vivo experiments showed that the tumor inhibition rate was dose-dependent to some extent; the best tumor inhibition rate was 64. 3% in the high dose group, followed by tumor inhibition rates of 46. 7% in the medium dose group and 33% in the low dose group. Spleen and thymus indexes in the EST group was significantly higher than those in the model group (P< 0. 05). Histological observation showed that the proliferation and invasion of cancer cells was inhibited to different degrees, and karyopyknosis of cancer cells was identified in the EST group. Conclusion EST could enhance the immune function of mice, induce apoptosis of tumor cells, and significantly inhibit the proliferation of carcinoma cells in a dose-dependent manner.
查看全文  查看/发表评论  下载PDF阅读器
关闭
您是第 1769975 位访问者
版权所有:中国实验动物学会 主管单位:中国科学技术协会 主办单位:中国实验动物学会    中国医学科学院医学实验动物研究所
地  址: 北京市朝阳区潘家园南里5号 邮编:100021 电话:010-67779337 E-mail:bjb2@cnilas.org
本系统由北京勤云科技发展有限公司设计
微信关注二维码