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舒晴,邵雨薇,田峻,杨亚南.大鼠孤束核微量注射的定位初探[J].中国实验动物学报,2020,28(4):429~435.
大鼠孤束核微量注射的定位初探
Preliminary investigation of localization of solitary nucleus microinjection in rats
投稿时间:2020-03-31  
DOI:10. 3969 / j.issn.1005-4847. 2020. 04. 001
中文关键词:  孤束核  脑立体定位术  脑微量注射
英文关键词:nucleus of the solitary tract  stereotaxy of brain  microinjection
基金项目:
作者单位E-mail
舒晴 武汉大学中南医院康复医学科,武汉 430071 shuqingj@ whu.edu.cn 
邵雨薇 1.武汉大学中南医院康复医学科,武汉 430071
2. 武汉大学第二临床学院,武汉 430071 
 
田峻 武汉大学中南医院康复医学科,武汉 430071  
杨亚南 3. 华润武钢总医院中医科,武汉 430080
4. 武汉科技大学职业危害识别与控制湖北省重点实验室,武汉 430000 
yangyn_wg@ 126.com 
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中文摘要:
      目的 通过应用不同参数定位注射,探讨大鼠孤束核微量注射的定位方法以提高注射成功率及准 确性。 方法 将 15 只大鼠根据实验进度的不同分为垂直注射组、Bregma 定位组和 Lambda 定位组。 垂直注射组进 针点选择为大鼠颅底前缘 0. 5 mm,进针深度 7. 5 mm;Bregma 定位组以 Bregma-11. 0 mm、双侧中线旁开 0. 5 ~ 0. 7 mm 为进针点,进针斜刺角度向后 24°,斜刺深度 8. 6 mm 为注射点;Lambda 定位组以 Lambda-3. 2 mm、双侧中线旁 开 0. 5 ~ 0. 7 mm 为进针点,进针斜刺角度向后 24°、斜刺深度 9. 6 mm 为注射点。 观察各组大鼠注射后全脑冠状切面的荧光标记位置。 结果 垂直注射组荧光标记位置位于脉络丛所在冠状切面。 Bregma 定位组荧光标记不稳定, 分别位于小脑、脑干较浅位置或脉络丛所在冠状切面。 Lambda 定位组中体重 300 g 左右的大鼠荧光标记位置位于 小脑和脑干的间隙,而体重 400 g 左右的大鼠能够准确标记于孤束核。 结论 在 400 g 左右的大鼠中,以 Lambda 为 基准点以一定角度斜刺可以准确注射至大鼠孤束核。
英文摘要:
      Objective To investigate the localization method of microinjection of the solitary nucleus in rats using different parameters and to improve its success rate and accuracy. Methods Twenty rats were divided into a vertical injection group, a Bregma-based localization group, and a Lambda-based localization group according to the experimental progress. The injection point of the vertical injection group was 0. 5 mm at the anterior edge of the skull base of the rat, with a needle insertion depth of 7. 5 mm. The Bregma positioning group used Bregma-11. 0 mm and 0. 5 - 0. 7 mm beside the bilateral midline as the puncture point. The oblique insertion angle was 24° backwards and the depth was 8. 6 mm at the injection point. The Lambda positioning group used Lambda-3. 2 mm and 0. 5 - 0. 7 mm beside the bilateral midline as the puncture point. The oblique insertion angle was 24° backwards and depth was 9. 6 mm at the injection point. The positions of the fluorescent markers in the coronal section of the whole brain after injection of each group of rats were observed. Results The fluorescent label in the vertical injection group was located in the choroid plexus. The fluorescent labeling was unstable in the Bregma-based localization group, and was located in the coronal sections of the cerebellum, superficial brainstem, and choroid plexus. In the Lambda-based localization group, the fluorescent label position in the rats weighing approximately 300 g was located in the space between the cerebellum and the brainstem, while the rats weighing around 400 g could be accurately labeled in the solitary nucleus. Conclusions In rats of approximately 400 g, oblique puncture at a certain angle based on Lambda as the reference point can enable accurate injection into the nucleus of the solitary tract in rats.
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