Drug toxicity has, in significant measure, affected the discovery and application of new pharmaceutical drugs. Zebrafish have been increasingly used as a biological model in drug toxicology research. Here, we reviewed the application of the zebrafish model in drug toxicity research in a number of fields ( including cardiotoxicity, hepatotoxicity, nephrotoxicity, neurotoxicity, genetic and intergenerational toxicity, and teratogenic toxicity). The relative advantages and disadvantages were summarized, thus providing a basis for further application of the zebrafish model in drug toxicity research.