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林晓.黄芪三七合剂通过调控 Arid2-IR/ NF-κB 信号轴改善糖尿病肾病小鼠肾炎症反应[J].中国实验动物学报,2020,28(3):382~389.
黄芪三七合剂通过调控 Arid2-IR/ NF-κB 信号轴改善糖尿病肾病小鼠肾炎症反应
Astragalus propinquus Schischkin and Panax notoginseng compound improves kidney inflammatory reactions in mice with diabetic nephropathy via regulation of Arid2-IR / NF-κB signaling
投稿时间:2019-11-20  
DOI:10. 3969 / j.issn.1005-4847. 2020. 03. 014
中文关键词:  糖尿病肾病  黄芪三七合剂  长链非编码 RNA  Arid2-IR  NF-κB
英文关键词:diabetic nephropathy  Astragalus propinquus Schischkin and Panax notoginseng compound  lncRNA  Arid2-IR  NF-κB
基金项目:
作者单位E-mail
林晓 西南医科大学附属中医医院中西医结合研究中心,四川 泸州 646000 1090388741@ qq.com 
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中文摘要:
      目的 研究黄芪三七合剂对糖尿病肾病(DN)小鼠的治疗作用并探讨潜在调控机制。 方法 50 只 C57BL/ 6 雄性小鼠随机分为对照组(NC 组)、模型组(DN 组)、黄芪三七合剂低/ 高剂量组(L-A&P,H-A&P 组)和厄 贝沙坦组(IRB 组)。 NC 组给予普通饲料,其余组均高脂高糖饲料喂养 2 月后,腹腔连续 5 d 注射链脲佐菌素 50 mg / (kg·d)以诱导糖尿病肾病模型,建模后给予不同灌胃处理,治疗 4 周后处死小鼠,HE、PAS 观察肾病理,检测肾 功能及小鼠肾组织中炎症因子(TNF-α、IL-1β、IL-6)、lncRNA Arid2-IR、NF-κB 及其下游信号分子的表达。 结果 与 DN 组相比,A&P 治疗后的小鼠 24 h 尿蛋白、血清肌酐和尿素氮明显下降,肾功能在一定程度上得到恢复;肾病 理结构明显好转,炎症因子( TNF-α、IL-1β、IL-6) 的表达呈现剂量依赖性下降,Arid2-IR 和 NF-κB 及其下游分子 (COX2,IL-6)的表达下调,表明 NF-κB 信号通路活化受到抑制。 结论 A&P 可有效减轻 DN 小鼠肾炎症反应,其 机制可能与调节 Arid2-IR/ NF-κB 信号轴有关。
英文摘要:
      Objective To explore the therapeutic effect and potential mechanism of action of Astragalus propinquus Schischkin and Panax notoginseng ( A&P ) compound in a mouse model of diabetic nephropathy ( DN). Methods Fifty male C57BL/ 6 mice were randomly divided into normal, model, L-A&P, H-A&P and irbesartan groups. Mice were fed a high-fat, high-sugar diet for 2 months, and streptozotocin 50 mg / (kg·d) was intraperitoneally injected for 5 days to induce DN. Different gavage treatments were given to the groups. After 4 weeks of treatment, mice were killed and materials collected. Renal pathology was observed using hematoxylin-eosin and periodic acid – Schiff stained sections. Renal function and the expression of Arid2-IR lncRNA, inflammatory factors [ tumor necrosis factor-α ( TNF-α), interleukin-1β (IL-1β), IL-6], and NF-κB and its downstream signaling molecules were examined. Results The 24 h urine protein, serum creatinine, and urea nitrogen levels were significantly decreased in A&P-treated mice compared with the DN group, which suggests that renal function recovered to a certain extent. Renal pathology was significantly improved, and IL-1β, IL-6 and TNF-α expression decreased in a dose-dependent manner. Expression levels of Arid2-IR and NF-κB and its downstream molecules ( COX2, IL-6) were down-regulated, indicating that activation of the NF-κB signaling pathway was inhibited. Conclusions A&P reduced kidney inflammation in mice with DN, and its mechanism of action may be related to the regulation of Arid2-IR/ NF-κB signaling.
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