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黎娅.长期稳定的 SD 大鼠 2 型糖尿病模型制备方法[J].中国实验动物学报,2020,28(3):364~369.
长期稳定的 SD 大鼠 2 型糖尿病模型制备方法
A long-term and stable method for the preparation of a type 2 diabetes Sprague-Dawley rat model
投稿时间:2019-11-24  
DOI:10. 3969 / j.issn.1005-4847. 2020. 03. 011
中文关键词:  2 型糖尿病  大鼠模型  链脲佐菌素  高脂饮食
英文关键词:type 2 diabetes mellitus  rat model  streptozotocin  high fat diet
基金项目:
作者单位E-mail
黎娅 青海大学附属医院,西宁 810001 864755431@ qq.com 
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中文摘要:
      目的 探讨高脂饮食联合链脲佐菌素(streptozotocin,STZ)诱导糖尿病大鼠模型的制备方法,为临床研究提供一种稳定的糖尿病动物模型。 方法 30 只雄性 SD 大鼠随机分成 2 组:正常对照组(NG,n = 10)普通饲料喂养;2 型糖尿病组(DM,n = 20)高脂饮食(high fat diet,HFD)喂养 12 周+STZ 25 mg / kg 尾静脉注射。 实验期间每 周检测大鼠体重,STZ 注射前 1 周观察大鼠口服糖耐量试验(OGTT)、胰岛素耐量试验( ITT)及血糖曲线下面积变 化。 STZ 注射后观察大鼠一般情况(摄食、饮水量、毛发光泽、垫料潮湿度及精神状况)、随机检测大鼠血糖、果糖胺。 结果 与 NG 组相比,DM 组大鼠体重增幅大,于喂养 12 周差异显著(P < 0. 05)。 OGTT 实验 60、90、120 min 血 糖明显高于 NG 组(P < 0. 05),曲线下面积显著增大(P < 0. 05);IPITT 试验 DM 组血糖明显高于 NG 组(P < 0. 05), 曲线下面积显著增大(P < 0. 05)。 STZ 干预后,两组大鼠摄食、饮水、体重显著差异(P < 0. 05),DM 组随机血糖及 果糖胺明显高于 NG 组(P < 0. 05)。 结论 高脂喂养大鼠 12 周后引起了胰岛素抵抗和糖耐量异常,再联合小剂量 STZ 诱导的 2 型糖尿病大鼠模型血糖稳定,是研究 2 型糖尿病的理想动物模型。
英文摘要:
      Objective To study a diabetic rat model induced by high-fat diet ( HFD) combined with streptozotocin (STZ), and to provide a stable diabetic animal model for clinical research. Methods Thirty male SD rats were randomly divided into two groups: normal control group (NG, n = 10) fed a normal diet; type 2 diabetes group (DM, n = 20) fed a HFD for 12 weeks + 25 mg STZ/ kg via tail vein injection. The body weight of rats was measured every week during the experiment. The oral glucose tolerance test, insulin tolerance test and the area change under the blood glucose curve were observed one week before STZ injection. After STZ injection, the general condition ( food intake, water consumption, hair luster, moisture and state of padding ), random blood glucose, fructosamine and pancreatic histopathological sections were examined. Results The body weight was significantly increased ( P < 0. 05) in the DM group compared with the NG group. The blood glucose was significantly higher in the oral glucose tolerance test group compared with that of the NG group ( P < 0. 05) at 60, 90 and 120 min, and the area under the curve was also significantly increased (P < 0. 05); blood glucose in the DM group was significantly higher than that of NG group (P < 0. 05), and the area under the curve was also significantly increased ( P < 0. 05). After STZ intervention, there were significant differences in food intake, diet and body weight between the two groups (P < 0. 05). Conclusions After 12 weeks of a HFD, insulin resistance and glucose tolerance were abnormal in rats. The model of type 2 diabetes induced by low-dose STZ provides a valuable animal model for the study of type 2 diabetes.
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