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张帆.Ⅰ型干扰素受体缺失小鼠体外受精实验研究[J].中国实验动物学报,2020,28(3):351~357.
Ⅰ型干扰素受体缺失小鼠体外受精实验研究
In vitro fertilization using type I interferon receptor-deficient mice
投稿时间:2019-10-12  
DOI:10. 3969 / j.issn.1005-4847. 2020. 03. 009
中文关键词:  I 型干扰素受体  小鼠  体外受精
英文关键词:type I interferon receptor  mice  in vitro fertilization
基金项目:
作者单位E-mail
张帆 中国科学院武汉病毒研究所,武汉 430071 zhangf@ wh.iov.cn 
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中文摘要:
      目的 探索两种 I 型干扰素受体缺失对于小鼠体外受精的影响并对体外受精条件进行优化。 方法 对两种 I 型干扰素受体缺失小鼠(IFN-α R- / - 、IFN-α/ β Rsup>- / - )及背景野生型小鼠(C57BL/ 6)分别进行体外受精及胚胎移植,每组超排 5 只小鼠,3 次重复,记录相关数据,分析干扰素受体缺失是否对小鼠体外受精存在影响。分别将 两种 I 型干扰素受体缺失小鼠配子与 C57BL/ 6 小鼠配子进行体外受精杂交试验,每组 5 只小鼠,3 次重复,探讨 I 型干扰素受体缺失对雌雄配子体外受精的影响。 同时,优化体外受精条件,探索提高受精率技术方法,每组 5 只小鼠,三次重复。 结果 两种 I 型干扰素受体缺失小鼠平均体外受精率低于背景品系 C57BL/ 6 小鼠,组间差异具有显著性(P < 0. 05)。 干扰素 α 受体缺失小鼠体外受精率高于干扰素 α、β 受体双缺失小鼠体外受精率,组间差异具有显著性(P < 0. 05)。 两种 I 型干扰素受体缺失小鼠的精子与 C57BL/ 6 小鼠卵细胞体外受精率均高于其卵细胞与 C57BL/ 6 小鼠精子的体外受精率,组间差异具有显著性(P < 0. 05)。通过延长精子获能时间至 1 h 或在获能液及受精液中加入 1 mmol / L 还原型谷胱甘肽(GSH)能够提高体外体外受精率,相应组间差异具有显著性(P < 0. 05)。 结论 I 型干扰素受体缺失可能导致相应品系小鼠体外受精率降低,而且对卵细胞的影响较精子的影响更为显著, 通过适当延长精子获能时间或改变获能及受精液成分,能够提高体外受精率。
英文摘要:
      Objective To explore the effects of two types of type I interferon (IFN-I) receptor deletions upon the reproductive performance of mice. Methods In vitro fertilization (IVF) was performed using two types of IFN-I receptor- deficient mice (dificient in IFN-α and IFN-α/ β receptor signaling) and wild-type background ( C57BL/ 6) mice. Each group contained 5 superovulated mice and the assay was repeated in triplicate. The female and male gametes of two the types of IFN receptor-deficient mice and the wild-type mice were examined using IVF. The IVF conditions were optimized and method to increase the fertilization rate were explored. Results The average IVF rates ( number of 2-cell embryos as a percentage of oocytes) for the two types of IFN receptor-deficient mice were significantly lower (P < 0. 05) than that of wild-type mice. The IVF rate of mice with IFN-α receptor deficiency was higher ( P < 0. 05) than that of mice with IFN-α/ β receptor deficiency. The IVF rates using receptor-deficient sperm and C57BL/ 6 oocytes were significantly higher (P < 0. 05) than those using receptor-deficient oocytes and C57BL/ 6 sperm. The IVF rates were significantly increased (P < 0. 05) by extending the sperm capacitation time from 30 min to 1 h or adding glutathione to the capacitation and fertilization solutions form 0 mmol / L to1 mmol / L. Conclusions Deletion of the IFN-I receptor may reduce the fertility of mice, and the effect on oocytes appears more significant than that upon sperm. Extending the sperm capacitation time or changing the capacitation and fertilization fluid composition improved the fertilization rate.
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